Recombinant Human TNFSF18 protein, N-hFc-Flag Tag

Product Description

Cat

IMP-6031

Official Symbol

TNFSF18

Product Overview

Recombinant Human TNFSF18 protein(Q9UNG2)(72-199aa), fused with N-terminal hFc-Flag tag, was expressed in HEK293.

Expression System

HEK293

Species

Human

Tag

N-hFc-Flag Tag

Form

Lyophilized from a 0.2 μm filtered PBS, 6% Trehalose, pH 7.4

Molecular Mass

42.8 kDa

Protein length

72-199aa

AA Sequence

QLETAKEPCMAKFGPLPSKWQMASSEPPCVNKVSDWKLEILQNGLYLIYGQVAPNANYNDVAPFEVRLYKNKDMIQTLTNKSKIQNVGGTYELHVGDTIDLIFNSEHQVLKNNTYWGIILLANPQFIS

Endotoxin

Less than 1.0 EU/ug as determined by LAL method.

Purity

Greater than 94% as determined by SDS-PAGE.

Biological Activity

①Measured by its binding ability in a functional ELISA. Immobilized TNFRSF18 at 2 μg/ml can bind TNFSF18, the EC50 is 2.565 to 2.940 ng/ml.②Human TNFRSF18 protein hFc tag captured on COOH chip can bind Human TNFSF18 protein hFc and Flag tag with an affinity constant of 38.5 nM as detected by LSPR Assay.

Storage

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

SDS-PAGE

Bioactivity-ELISA 1

Measured by its binding ability in a functional ELISA. Immobilized TNFRSF18 at 2 ug/ml can bind TNFSF18, the EC50 is 2.565 to 2.940 ng/ml

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

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