Recombinant Human TNFSF18 protein, N-His Tag

Product Description

Cat

IMP-119

Official Symbol

TNFSF18

Product Overview

Recombinant Human TNFSF18 protein(AA Gln 50-Ser 177)(Accession # AAH69319.1) is expressed from human 293 cells (HEK293). This protein carries a polyhistidine tag at the N-terminus.

Expression System

HEK293

Species

Human

Tag

N-His Tag

Form

Lyophilized from sterile PBS, pH7.4, 10% trehalose.

Molecular Mass

This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 20.5 kDa. The protein migrates as 23 kDa and 26 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Protein length

Gln 50-Ser 177

Endotoxin

Less than 1.0 EU per ug by the LAL method.

Purity

>90% as determined by SDS-PAGE.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4ºC before opening to recover the entire contents.

SDS-PAGE

Human GITR Ligand, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity-ELISA 1

Immobilized Human GITR Ligand, His Tag at 2 ug/mL (100 uL/well) can bind Human GITR, Fc Tag with a linear range of 0.1-4 ng/mL.

Bioactivity-ELISA 2

Immobilized Human GITR Ligand, His Tag at 2 ug/mL (100 uL/well) can bind Human GITR, Mouse IgG2a Fc Tag, low endotoxin with a linear range of 0.2-8 ng/mL.

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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