Recombinant Human TNFSF4 protein, C-Fc Tag

Product Description

Cat

IMP-056

Official Symbol

TNFSF4

Product Overview

Recombinant Human TNFSF4 protein(AA Leu 29-Ala 216)(Accession # NP_003318) is expressed from human 293 cells (HEK293). This protein carries a human IgG1 Fc tag at the C-terminus.

Expression System

HEK293

Species

Human

Tag

C-Fc Tag

Form

Lyophilized from sterile Tris with Glycine, Arginine and NaCl, pH7.5, 10% trehalose.

Molecular Mass

This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 46.8 kDa. The protein migrates as 60-65 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Protein length

Leu 29-Ala 216

Endotoxin

Less than 1.0 EU per ug by the LAL method.

Purity

>90% as determined by SDS-PAGE.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4ºC before opening to recover the entire contents.

SDS-PAGE

Human OX40, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity-ELISA 1

Immobilized Human OX40 Ligand, His Tag (active trimer) (HPLC-verified) at 5 ug/mL (100 uL/well) can bind Human OX40, Fc Tag with a linear range of 2-25 ng/mL.

Bioactivity-ELISA 2

Quantitative Analysis of CTLA-4 x OX40 Bispecific Antibody in Human Serum by Intact Assay. Immobilized Human OX40, Fc Tag at 2 ug/mL, add increasing concentrations of CTLA-4 x OX40 Bispecific Antibody in 50% Human serum and then add Biotinylated Human CTLA-4, Fc,Avitag at 0.2 ug/mL. Detection was performed using HRP-conjugated streptavidin with sensitivity of 2 ng/mL (Intact assay, Routinely tested).

Bioactivity-FACS 1

FACS assay shows that recombinant Human OX40, Fc Tag can bind to 293T cell overexpressing human OX40L. The concentration of OX40 used is 0.03 ug/ml.

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

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