Human OX40 Protein, His Tag (MALS verified) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
The purity of Human OX40, His tag (MALS verified) was more than 85% and the molecular weight of this protein is around 32-44 kDa verified by SEC-MALS.
Immobilized Human OX40, His Tag at 2 ug/mL (100 uL/well) can bind Human OX40 Ligand, Fc Tag with a linear range of 0.1-3 ng/mL.
Immobilized Human OX40, His Tag at 2 ug/mL (100 uL/well) can bind Monoclonal Anti-Human OX40 Antibody, Human IgG1 with a linear range of 0.1-2 ng/mL.
Quantitative Analysis of CTLA-4 x OX40 Bispecific Antibody in Human Serum by Intact Assay. Immobilized Human OX40 Protein, His Tag (MALS verified) at 2 ug/mL, add increasing concentrations of CTLA-4 x OX40 bispecific antibody in 50% Human serum and then add Biotinylated Human CTLA-4, Fc,Avitag at 0.2 ug/mL. Detection was performed using HRP-conjugated streptavidin with sensitivity of 4 ng/mL (Intact assay, Routinely tested).
Loaded Human OX40 Ligand, His Tag (active trimer) on AR2G Biosensor, can bind Human OX40, His Tag with an affinity constant of 0.15 uM as determined in BLI assay (ForteBio Octet Red96e).
Loaded Anti-OX40 MAb(Human IgG1) on AHC Biosensor, can bind Human OX40, His Tag with an affinity constant of 6.03 nM as determined in BLI assay (ForteBio Octet Red96e).