Recombinant Human TNFSF4 protein, C-Fc Tag

Product Description

Cat

IMP-057

Official Symbol

TNFSF4

Product Overview

Human OX40, His Tag (OX0-H5224) is expressed from human 293 cells (HEK293). It contains AA Leu 29-Ala 216 (Accession # P43489-1). This protein carries a polyhistidine tag at the C-terminus.

Expression System

HEK293

Species

Human

Tag

C-His Tag

Form

Lyophilized from sterile PBS, pH7.4, 10% trehalose.

Molecular Mass

This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 22.0 kDa. The protein migrates as 40-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Protein length

Leu 29-Ala 216

Endotoxin

Less than 1.0 EU per ug by the LAL method.

Purity

>90% as determined by SDS-PAGE.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4ºC before opening to recover the entire contents.

SDS-PAGE

Human OX40 Protein, His Tag (MALS verified) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

SEC-MALS 1

The purity of Human OX40, His tag (MALS verified) was more than 85% and the molecular weight of this protein is around 32-44 kDa verified by SEC-MALS.

Bioactivity-ELISA 1

Immobilized Human OX40, His Tag at 2 ug/mL (100 uL/well) can bind Human OX40 Ligand, Fc Tag with a linear range of 0.1-3 ng/mL.

Bioactivity-ELISA 2

Immobilized Human OX40, His Tag at 2 ug/mL (100 uL/well) can bind Monoclonal Anti-Human OX40 Antibody, Human IgG1 with a linear range of 0.1-2 ng/mL.

Bioactivity-ELISA 3

Quantitative Analysis of CTLA-4 x OX40 Bispecific Antibody in Human Serum by Intact Assay. Immobilized Human OX40 Protein, His Tag (MALS verified) at 2 ug/mL, add increasing concentrations of CTLA-4 x OX40 bispecific antibody in 50% Human serum and then add Biotinylated Human CTLA-4, Fc,Avitag at 0.2 ug/mL. Detection was performed using HRP-conjugated streptavidin with sensitivity of 4 ng/mL (Intact assay, Routinely tested).

Bioactivity-BLI 1

Loaded Human OX40 Ligand, His Tag (active trimer) on AR2G Biosensor, can bind Human OX40, His Tag with an affinity constant of 0.15 uM as determined in BLI assay (ForteBio Octet Red96e).

Bioactivity-BLI 2

Loaded Anti-OX40 MAb(Human IgG1) on AHC Biosensor, can bind Human OX40, His Tag with an affinity constant of 6.03 nM as determined in BLI assay (ForteBio Octet Red96e).

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

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