Recombinant Human TNFSF4 protein, C-mFc Tag

Product Description

Cat

IMP-009

Official Symbol

TNFSF4

Product Overview

Recombinant Human TNFSF4 protein(AA Gln 51-Leu 183)(Accession # P23510-1) is expressed from human 293 cells (HEK293). This protein carries a mouse IgG2a Fc tag at the C-terminus.

Expression System

HEK293

Species

Human

Tag

C-mFc Tag

Form

Lyophilized from sterile PBS, pH7.4, 10% trehalose.

Molecular Mass

This protein carries a mouse IgG2a Fc tag at the C-terminus. The protein has a calculated MW of 42.3 kDa. The protein migrates as 53-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Protein length

Gln 51-Leu 183

Endotoxin

Less than 0.1 EU per ug by the LAL method.

Purity

>95% as determined by SDS-PAGE.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4ºC before opening to recover the entire contents.

Bioactivity-FACS Title 2

Bioactivity-FACS

SDS-PAGE

Human OX40 Ligand, Mouse IgG2a Fc Tag, low endotoxin on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA 1

Immobilized Human OX40, Fc Tag at 0.5 ug/mL (100 uL/well) can bind Human OX40 Ligand, Mouse IgG2a Fc Tag, low endotoxin with a linear range of 0.8-13 ng/mL.

Bioactivity-BLI 1

Loaded Human OX40 Ligand, Mouse IgG2a Fc Tag, low endotoxin on Protein A Biosensor, can bind Human OX40, His Tag with an affinity constant of 2 uM as determined in BLI assay (ForteBio Octet Red96e).

Bioactivity-FACS 1

FACS assay shows that recombinant Human OX40 Ligand, Mouse IgG2a Fc Tag, low endotoxin can bind to 293T cell overexpressing human OX40. The concentration of OX40 Ligand is 0.003 ug/ml.

Bioactivity-FACS 2

FACS analysis shows that the binding of Human OX40 Ligand, Mouse IgG2a Fc Tag, low endotoxin to 293T overexpressing OX40 was inhibited by increasing concentration of neutralizing anti-OX40 antibody. The concentration of OX40 Ligand used is 0.002 ug/ml. IC50=3.021 ug/ml.

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

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