Recombinant Human TNFSF4 protein, C-mFc Tag

Product Description

Cat

IMP-009

Official Symbol

TNFSF4

Product Overview

Recombinant Human TNFSF4 protein(AA Gln 51-Leu 183)(Accession # P23510-1) is expressed from human 293 cells (HEK293). This protein carries a mouse IgG2a Fc tag at the C-terminus.

Expression System

HEK293

Species

Human

Tag

C-mFc Tag

Form

Lyophilized from sterile PBS, pH7.4, 10% trehalose.

Molecular Mass

This protein carries a mouse IgG2a Fc tag at the C-terminus. The protein has a calculated MW of 42.3 kDa. The protein migrates as 53-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Protein length

Gln 51-Leu 183

Endotoxin

Less than 0.1 EU per ug by the LAL method.

Purity

>95% as determined by SDS-PAGE.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4ºC before opening to recover the entire contents.

Bioactivity-FACS Title 2

Bioactivity-FACS

SDS-PAGE

Human OX40 Ligand, Mouse IgG2a Fc Tag, low endotoxin on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA 1

Immobilized Human OX40, Fc Tag at 0.5 ug/mL (100 uL/well) can bind Human OX40 Ligand, Mouse IgG2a Fc Tag, low endotoxin with a linear range of 0.8-13 ng/mL.

Bioactivity-BLI 1

Loaded Human OX40 Ligand, Mouse IgG2a Fc Tag, low endotoxin on Protein A Biosensor, can bind Human OX40, His Tag with an affinity constant of 2 uM as determined in BLI assay (ForteBio Octet Red96e).

Bioactivity-FACS 1

FACS assay shows that recombinant Human OX40 Ligand, Mouse IgG2a Fc Tag, low endotoxin can bind to 293T cell overexpressing human OX40. The concentration of OX40 Ligand is 0.003 ug/ml.

Bioactivity-FACS 2

FACS analysis shows that the binding of Human OX40 Ligand, Mouse IgG2a Fc Tag, low endotoxin to 293T overexpressing OX40 was inhibited by increasing concentration of neutralizing anti-OX40 antibody. The concentration of OX40 Ligand used is 0.002 ug/ml. IC50=3.021 ug/ml.

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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