Recombinant Human TNFSF4 Protein (Gln51-Leu183), N-10×His-tagged

Product Description

Cat

IMP-9604

Official Symbol

TNFSF4

Product Overview

Recombinant human OX40 Ligand/TNFSF4 protein (Gln51-Leu183) with a 10-His tag at N-terminus was expressed in Mouse myeloma cell line.

Description

This gene encodes a cytokine of the tumor necrosis factor (TNF) ligand family. The encoded protein functions in T cell antigen-presenting cell (APC) interactions and mediates adhesion of activated T cells to endothelial cells. Polymorphisms in this gene have been associated with Sjogren's syndrome and systemic lupus erythematosus. Alternative splicing results in multiple transcript variants.

Expression System

Mouse myeloma cell line

Species

Human

Tag

N-10×His

Predicted N Terminal

Met

Form

Supplied as a 0.2 μm filtered solution in PBS.

Molecular Mass

Predicted Molecular Mass: 18 kDa
SDS-PAGE: 28-34 kDa, reducing conditions

Protein length

Gln51-Leu183

Bio-activity

Measured by its ability to induce IL-8 secretion in HT1080 human fibrosarcoma cells transfected with human OX40/TNFRSF4. Muller, N. et al. (2008) FEBS J. 275:2296. The ED50 for this effect is typically 1-5 ng/mL. Measured by its binding ability in a functional ELISA. When Recombinant Human OX40/TNFRSF4 Fc Chimera is immobilized at 10 ng/mL, the concentration of Recombinant Human OX40 Ligand/TNFSF4 that produces 50% optimal binding response is found to be approximately 0.5-3 ng/mL.

Endotoxin

<1.0 EU/μg of the protein by the LAL method.

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after opening.

SDS-PAGE

Bioactivity-ELISA 1

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

Online Inquiry