Human OX40 Ligand, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
Immobilized Human OX40, His Tag at 0.5 ug/mL (100 uL/well) can bind Human OX40 Ligand, Fc Tag with a linear range of 2-63 ng/mL.
Loaded Human OX40 Ligand, Fc Tag on Protein A Biosensor, can bind Human OX40, His Tag with an affinity constant of 0.96 uM as determined in BLI assay (ForteBio Octet Red96e).
FACS assay shows that recombinant Human OX40 Ligand, Fc Tag can bind to 293T cell overexpressing human OX40. The concentration of OX40 Ligand used is 0.01 ug/ml.