Recombinant Human TNFSF8 Protein (63-234aa), C-His-Tagged

Product Description

Cat

IMP-9391

Official Symbol

TNFSF8

Product Overview

Recombinant human TNFSF8, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Description

TNFSF8, also known as tumor necrosis factor ligand superfamily member 8, is a type II membrane protein belonging to the TNF superfamily. It is expressed on the cell surface of activated T cells, B cells, and monocytes. CD30/TNFRSF8 ligation by TNFSF8 mediates pleiotropic effects including cell proliferation, activation, differentiation and cell death by apoptosis.

Expression System

Baculovirus

Species

Human

Tag

C-His

Form

Liquid in Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Molecular Mass

20.7 kDa (181 aa)

Protein length

63-234

AA Sequence

<ADP>QRTDSIPNSPDNVPLKGGNCSEDLLCILKRAPFKKSWAYLQVAKHLNKTKLSWNKDGILHGVRYQDGNLVIQFPGLYFIICQLQFLVQCPNNSVDLKLELLINKHIKKQALVTVCESGMQTKHVYQNLSQFLLDYLQVNTTISVNVDTFQYIDTSTFPLENVLSIFLYSNSD<HHHHHH>

Endotoxin

< 1 EU/μg of protein (determined by LAL method)

Purity

> 85% by SDS-PAGE

Applications

SDS-PAGE, Bioactivity

Notes

Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.

Storage

Can be stored at +2 to +8 centigrade for 1 week. For long term storage, aliquot and store at -20 to -80 centigrade. Avoid repeated freezing and thawing cycles.

Concentration

0.5 mg/mL (determined by absorbance at 280nm)

SDS-PAGE

3 μg by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

Bioactivity-ELISA 1

Human CD30 Ligand/TNFSF8 is coated at 2 μg/mL (100 μL/well) can bind Human CD30/TNFRSF8 in a Functional ELISA assay.

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

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