Recombinant Human TNFSF8 Protein (63-234aa), C-His-Tagged

Product Description

Cat

IMP-9391

Official Symbol

TNFSF8

Product Overview

Recombinant human TNFSF8, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Description

TNFSF8, also known as tumor necrosis factor ligand superfamily member 8, is a type II membrane protein belonging to the TNF superfamily. It is expressed on the cell surface of activated T cells, B cells, and monocytes. CD30/TNFRSF8 ligation by TNFSF8 mediates pleiotropic effects including cell proliferation, activation, differentiation and cell death by apoptosis.

Expression System

Baculovirus

Species

Human

Tag

C-His

Form

Liquid in Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Molecular Mass

20.7 kDa (181 aa)

Protein length

63-234

AA Sequence

<ADP>QRTDSIPNSPDNVPLKGGNCSEDLLCILKRAPFKKSWAYLQVAKHLNKTKLSWNKDGILHGVRYQDGNLVIQFPGLYFIICQLQFLVQCPNNSVDLKLELLINKHIKKQALVTVCESGMQTKHVYQNLSQFLLDYLQVNTTISVNVDTFQYIDTSTFPLENVLSIFLYSNSD<HHHHHH>

Endotoxin

< 1 EU/μg of protein (determined by LAL method)

Purity

> 85% by SDS-PAGE

Applications

SDS-PAGE, Bioactivity

Notes

Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.

Storage

Can be stored at +2 to +8 centigrade for 1 week. For long term storage, aliquot and store at -20 to -80 centigrade. Avoid repeated freezing and thawing cycles.

Concentration

0.5 mg/mL (determined by absorbance at 280nm)

SDS-PAGE

3 μg by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

Bioactivity-ELISA 1

Human CD30 Ligand/TNFSF8 is coated at 2 μg/mL (100 μL/well) can bind Human CD30/TNFRSF8 in a Functional ELISA assay.

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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