Recombinant Human TNFSF9 protein, C-His Tag

Product Description

Cat

IMP-116

Official Symbol

TNFSF9

Product Overview

Recombinant Human TNFSF9 protein(AA Ala 50-Glu 254)(Accession # P41273-1) is expressed from human 293 cells (HEK293). This protein carries a polyhistidine tag at the C-terminus.

Expression System

HEK293

Species

Human

Tag

C-His Tag

Form

Lyophilized from sterile PBS with Arginine, pH7.4, 10% trehalose.

Molecular Mass

This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 23.3 kDa. The protein migrates as 25 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Protein length

Ala 50-Glu 254

Endotoxin

Less than 1.0 EU per ug by the LAL method.

Purity

>95% as determined by SDS-PAGE.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4ºC before opening to recover the entire contents.

SDS-PAGE

Human 4-1BB Ligand, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA 1

Immobilized Human 4-1BB, Mouse IgG2a Fc Tag, low endotoxin at 1 ug/mL (100 uL/well) can bind Human 4‑1BB Ligand, His Tag with a linear range of 0.1-2 ng/mL.

Bioactivity-BLI 1

Loaded Human 4-1BB, Fc Tag on Protein A Biosensor, can bind Human 4-1BB Ligand, His Tag with an affinity constant of 1.3 nM as determined in BLI assay (ForteBio Octet Red96e).

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

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