Recombinant Human TNFSF9 Protein, N-hFc-tagged

Product Description

Cat

IMP-1383

Official Symbol

TNFSF9

Product Overview

Recombinant human TNFSF9 (NP_003802.1) (Arg71-Glu254) was expressed with the Fc region of human IgG1 at the N-terminus.

Description

4-1BB ligand is the high affinity ligand of 4-1BB, also known as CD137L or TNFSF9. It is shown to be a type II surface glycoprotein belonging to the TNF superfamily. Expression of 4-1BBL is restricted to APCs, such as dendritic cells, macrophages, and activated B cells. Members of the TNF-TNF receptor superfamily have been shown to play critical roles in regulating cellular activation, differentiation and apoptosis. Several studies have reported that 4-1BBL/4-1BB interaction provided a co-stimulatory signal to T cells, and increased T cell proliferation and cytokines production. In addition, 4-1BBL is involved in cancers, infectious diseases and autoimmune diseases.

Expression System

HEK293

Species

Human

Tag

N-hFc

Predicted N Terminal

Glu

Form

Lyophilized from sterile PBS, pH 7.4, 5 % trehalose, 5% mannitol and 0.01% Tween80.

Molecular Mass

The recombinant human TNFSF9 consists 444 amino acids and predicts a molecular mass of 47.9 kDa.

Protein length

Arg71-Glu254

Endotoxin

< 1.0 EU/μg protein as determined by the LAL method.

Purity

> 85 % as determined by SDS-PAGE

Storage

Samples are stable for up to twelve months from date of receipt at -20 to -80 centigrade.
Store it under sterile conditions at -20 to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Reconstitution

A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

SDS-PAGE

Bioactivity-ELISA 1

Immobilized human TNFRSF9 at 10 μg/mL (100 μL/well) can bind human TNFSF9, the EC50 of human TNFSF9 is 7-40 ng/mL.

Bioactivity-Cell based assay 1

Measured by its ability to induce IL-8 secretion by HT1080 transfected with human CD137. The EC50 for this effect is typically 0.3-1.2 μg/mL.

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

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