Description
TP53 is the most important tumor suppressor in the genome. It is responsive to numerous genotoxic stresses, which activates its transcription factor activity, in turn causing cell-cycle arrest by activating expression of p21 Cip/WAF. Mutant p53 that has lost its DNA-binding function interferes with the activity of native p53 and leads to oncogenic transformation. Alternatively, transformation may be caused by overexpression of Mdm2/Hdm2, an ubiquitin ligase specific for p53, which causes its destabilization. Inactivation of p53 is often coincident with hyperactivation of NFkB (NFkB p50 and NFkB p65), both of which serve to inhibit apoptosis.
Form
Recombinant p53 protein in Dilution Buffer AM1 (20 mM Tris-Cl (pH 7.5), 20% glycerol, 100 mM KCl, 1 mM DTT and 0.2 mM EDTA).
