Recombinant Human ULBP1 protein, C-hFc-Myc Tag

Product Description

Cat

IMP-6012

Official Symbol

ULBP1

Product Overview

Recombinant Human ULBP1 protein(Q9BZM6)(26-216aa), fused with C-terminal hFc-Myc tag, was expressed in HEK293.

Expression System

HEK293

Species

Human

Tag

C-hFc-Myc Tag

Form

Lyophilized from a 0.2 μm filtered PBS, 6% Trehalose, pH 7.4

Molecular Mass

52.4 kDa

Protein length

26-216aa

AA Sequence

GWVDTHCLCYDFIITPKSRPEPQWCEVQGLVDERPFLHYDCVNHKAKAFASLGKKVNVTKTWEEQTETLRDVVDFLKGQLLDIQVENLIPIEPLTLQARMSCEHEAHGHGRGSWQFLFNGQKFLLFDSNNRKWTALHPGAKKMTEKWEKNRDVTMFFQKISLGDCKMWLEEFLMYWEQMLDPTKPPSLAPG

Endotoxin

Less than 1.0 EU/ug as determined by LAL method.

Purity

Greater than 93% as determined by SDS-PAGE.

Biological Activity

①Measured by its binding ability in a functional ELISA. Immobilized KLRK1 at 10 μg/ml can bind human ULBP1, the EC50 of human ULBP1 protein is 228.5-427.6 ng/ml.
②Human KLRK1 protein Fc tag captured on COOH chip can bind Human ULBP1 protein Fc/myc tag with an affinity constant of 2.27 nM as detected by LSPR Assay.

Storage

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

SDS-PAGE

Bioactivity-ELISA 1

Measured by its binding ability in a functional ELISA. Immobilized KLRK1 at 10 ug/ml can bind human ULBP1, the EC50 of human ULBP1 protein is 228.5-427.6 ng/ml

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

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