Recombinant Human ULBP1 Protein (Gly26-Pro215), C-Fc-tagged

Product Description

Cat

IMP-9682

Official Symbol

ULBP1

Product Overview

Recombinant human ULBP-1 protein (Gly26-Pro215) with a human IgG1 (Pro100-Lys330) Fc tag at C-terminus was expressed in Spodoptera frugiperda, Sf 21 (stably transfected) cells.

Description

The protein encoded by this gene is a ligand of natural killer group 2, member D (NKG2D), an immune system-activating receptor on NK cells and T-cells. Binding of the encoded ligand to NKG2D leads to activation of several signal transduction pathways, including those of JAK2, STAT5, ERK and PI3K kinase/Akt. Also, in cytomegalovirus-infected cells, this ligand binds the UL16 glycoprotein and is prevented from activating the immune system. Three transcript variants encoding different isoforms have been found for this gene.

Expression System

Sf 21 cells

Species

Human

Tag

C-Fc

Predicted N Terminal

Gly26

Form

Lyophilized from a 0.2 μm filtered solution in PBS.

Molecular Mass

Predicted Molecular Mass: 48.8 kDa (monomer)
SDS-PAGE: 55 kDa, reducing conditions

Protein length

Gly26-Pro215

Bio-activity

Measured by its ability to bind rhNKG2D/Fc Chimera in a functional ELISA.

Endotoxin

<0.10 EU/μg of the protein by the LAL method.

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Notes

Disulfide-linked homodimer

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.

Reconstitution

Reconstitute at 100 μg/mL in sterile PBS.

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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