Recombinant Human ULBP2 Protein (Gly26-Ser217), C-hFc and Avi-tagged, Biotinylated
Product Description
Cat
IMP-10063
Official Symbol
ULBP2
Product Overview
Recombinant human ULBP-2 protein (Gly26-Ser217) with a Human IgG1 (Pro100-Lys330) Fc tag and Avi-tag at C-terminus was expressed in Chinese Hamster Ovary cell line.
Description
This gene encodes a major histocompatibility complex (MHC) class I-related molecule that binds to the NKG2D receptor on natural killer (NK) cells to trigger release of multiple cytokines and chemokines that in turn contribute to the recruitment and activation of NK cells. The encoded protein undergoes further processing to generate the mature protein that is either anchored to membrane via a glycosylphosphatidylinositol moiety, or secreted. Many malignant cells secrete the encoded protein to evade immunosurveillance by NK cells. This gene is located in a cluster of multiple MHC class I-related genes on chromosome 6.
Expression System
CHO
Species
Human
Tag
C-hFc and Avi
Predicted N Terminal
Gly26
Form
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Conjugate
Biotinylated
Molecular Mass
Predicted Molecular Mass: 50 kDa
SDS-PAGE: 60-70 kDa, under reducing conditions.
Protein length
Gly26-Ser217
Bio-activity
Measured by its binding ability in a functional ELISA. When Recombinant Human NKG2D/CD314 Fc Chimera is immobilized at 0.5 μg/mL (100 μL/well), Biotinylated Recombinant Human ULBP-2 Fc Chimera Avi-tag binds with an ED50 of 0.500-8.00 ng/mL.
Endotoxin
<0.10 EU/μg of the protein by the LAL method.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.
Notes
Disulfide-linked homodimer, biotinylated via Avi-tag
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 250 μg/mL in PBS.
SDS-PAGE
SDS-PAGE

Bioactivity-ELISA 1
Binding Activity

Measured by its binding ability in a functional ELISA. When Recombinant Human NKG2D/CD314 Fc Chimera is immobilized at 0.5 μg/mL (100 μL/well), Biotinylated Recombinant Human ULBP-2 Fc Chimera Avi-tag binds with an ED50 of 0.500-8.00 ng/mL.

Data Sheet MSDS
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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