Recombinant Human VCAM1 Protein (Phe25-Glu698)
Product Description
Cat
IMP-9910
Official Symbol
VCAM1
Product Overview
Recombinant human VCAM-1/CD106 protein (Phe25-Glu698) with a substitution of Arg322Gly and an N-terminal Met was expressed in Mouse myeloma cell line.
Description
This gene is a member of the Ig superfamily and encodes a cell surface sialoglycoprotein expressed by cytokine-activated endothelium. This type I membrane protein mediates leukocyte-endothelial cell adhesion and signal transduction, and may play a role in the development of artherosclerosis and rheumatoid arthritis. Three alternatively spliced transcripts encoding different isoforms have been described for this gene.
Expression System
Mouse myeloma cell line
Species
Human
Tag
Tag Free
Predicted N Terminal
Met
Form
Lyophilized from a 0.2 μm filtered solution in PBS containing Calcium and Magnesium with Sorbitol.
Molecular Mass
Predicted Molecular Mass: 74 kDa
SDS-PAGE: 90-95 kDa, reducing conditions
Protein length
Phe25-Glu698
Bio-activity
Measured by the ability of the immobilized protein to support the adhesion of U937 human histiocytic lymphoma cells. When 5 × 10^4 cells/well are added to human VCAM-1 coated plates (10 μg/mL with 100 μL/well), >85% cells will adhere after 1 hour incubation at 37 centigrade. Optimal dilutions should be determined by each laboratory for each application.
Endotoxin
<1.0 EU/μg of the protein by the LAL method.
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 1 mg/mL in sterile, deionized water.
Data Sheet MSDS
For research or industrial raw materials, not for personal medical use!

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