Recombinant Human VEGFA(VEGF121) protein

Product Description

Cat

IMP-623

Official Symbol

VEGFA

Product Overview

Recombinant Human VEGFA protein(NP_001020537.2)(Ala 27-Arg 147) was expressed in HEK293.

Expression System

HEK293

Species

Human

Form

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Molecular Mass

The protein has a calculated MW of 14.1 kDa. As a result of glycosylation, the protein migrates as 19 kDa and 20 kDa under reducing (R) condition, and 40-45 kDa (homodimer) under non-reducing (NR) condition (SDS-PAGE).

Protein length

Ala 27-Arg 147

Endotoxin

Less than 1.0 EU per μg by the LAL method.

Purity

>95% as determined by SDS-PAGE.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

SDS-PAGE

Bioactivity-ELISA 1

Immobilized ActiveMax Human VEGF121, Tag Free at 2 μg/mL (100 μL/well) can bind Human VEGF R2, Strep Tag with a linear range of 0.15-2.5 μg/mL.

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

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