Recombinant Human VEGFA(VEGF121) protein, N-His-Avi Tag, Biotinylated
Product Description
Cat
IMP-622
Official Symbol
VEGFA
Product Overview
Biotinylated Recombinant Human VEGFA protein(NP_001020537.2)(Ala 27-Arg 147), fused to N-His-Avi Tag, was expressed in HEK293.
Expression System
HEK293
Species
Human
Tag
N-His-Avi Tag, Biotinylated
Form
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Molecular Mass
The protein has a calculated MW of 16.7 kDa. As a result of glycosylation, the protein migrates as 18 kDa and 22 kDa under reducing (R) condition, and 35-40 kDa under non-reducing (NR) condition (SDS-PAGE).
Protein length
Ala 27-Arg 147
Endotoxin
Less than 1.0 EU per μg by the LAL method.
Purity
>95% as determined by reduced SDS-PAGE.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
Reconstitution
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
SDS-PAGE
SDS-PAGE

Bioactivity-ELISA 1
Bioactivity-ELISA

Immobilized VEGFR1/R2-Fc at 1 μg/mL (100 μL/well) can bind Biotinylated Human VEGF121, Avitag,His Tag with a linear range of 0.1-2 ng/mL.

Data Sheet MSDS
For research or industrial raw materials, not for personal medical use!

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