Recombinant Human VEGFA(VEGF121) protein, N-His-Avi Tag, Biotinylated

Product Description

Cat

IMP-622

Official Symbol

VEGFA

Product Overview

Biotinylated Recombinant Human VEGFA protein(NP_001020537.2)(Ala 27-Arg 147), fused to N-His-Avi Tag, was expressed in HEK293.

Expression System

HEK293

Species

Human

Tag

N-His-Avi Tag, Biotinylated

Form

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Molecular Mass

The protein has a calculated MW of 16.7 kDa. As a result of glycosylation, the protein migrates as 18 kDa and 22 kDa under reducing (R) condition, and 35-40 kDa under non-reducing (NR) condition (SDS-PAGE).

Protein length

Ala 27-Arg 147

Endotoxin

Less than 1.0 EU per μg by the LAL method.

Purity

>95% as determined by reduced SDS-PAGE.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

SDS-PAGE

Bioactivity-ELISA 1

Immobilized VEGFR1/R2-Fc at 1 μg/mL (100 μL/well) can bind Biotinylated Human VEGF121, Avitag,His Tag with a linear range of 0.1-2 ng/mL.

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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