Recombinant Human VEGFA(VEGF165) protein

Product Description

Cat

IMP-619

Official Symbol

VEGFA

Product Overview

Recombinant Human VEGFA protein(NP_001020537.2)(Ala 27-Arg 191) was expressed in HEK293.

Expression System

HEK293

Species

Human

Form

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Molecular Mass

The protein has a calculated MW of 19.2 kDa. As a result of glycosylation, the protein migrates as 24 kDa (monomer) under reducing (R) condition, and 43-50 kDa (homodimer) under non-reducing (NR) condition (SDS-PAGE).

Protein length

Ala 27-Arg 191

Endotoxin

Less than 1.0 EU per μg by the LAL method.

Purity

>98% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 24 months under sterile conditions after reconstitution.

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

SDS-PAGE

SEC-MALS 1

Bioactivity-ELISA 1

Immobilized ActiveMax Human VEGF165, Tag Free (MALS verified) at 0.01 μg/mL (100 μL/well) can bind Human VEGF R1, His Tag with a linear range of 4-31 ng/mL.

Bioactivity-ELISA 2

Immobilized ActiveMax Human VEGF165, Tag Free (MALS verified) at 2 μg/mL (100 μL/well) can bind Biotinylated Human VEGF R2, Avitag,His Tag with a linear range of 10-156 ng/mL.

Bioactivity-ELISA 3

Immobilized ActiveMax Human VEGF165, Tag Free (MALS verified) at 2 μg/mL (100 μL/well) can bind pre-mixed increasing concentrations of Bevacizumab and 0.5 μg/mL (100 μL/well) Biotinylated Human VEGF R2, Avitag,His Tag with a half maximal inhibitory concentration (IC50) of 0.70 μg/mL.

Bioactivity-BLI 1

Loaded Avastin (Bevacizumab) on AHC Biosensor, can bind ActiveMax Human VEGF165, Tag Free (MALS verified) with an affinity constant of 0.016 nM as determined in BLI assay.

Bioactivity-SPR 1

Avastin (Bevacizumab) captured on CM5 chip via anti-human IgG Fc antibodies surface, can bind ActiveMax Human VEGF165, Tag Free (MALS verified) with an affinity constant of 0.776 nM as determined in a SPR assay.

Bioactivity-Cell based assay 1

ActiveMax Human VEGF165, Tag Free (MALS verified) stimulates proliferation of human umbilical vein endothelial cells (HUVEC). The ED50 for this effect is 4.216-9.281 ng/mL.

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

Online Inquiry