Recombinant Human VEGFA(VEGF165) protein, N-His Tag

Product Description

Cat

IMP-621

Official Symbol

VEGFA

Product Overview

Recombinant Human VEGFA protein(NP_001020537.2)(Ala 27-Arg 191), fused to N-His Tag, was expressed in HEK293.

Expression System

HEK293

Species

Human

Tag

N-His Tag

Form

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Molecular Mass

The protein has a calculated MW of 21.1 kDa. The protein migrates as 25-27 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Protein length

Ala 27-Arg 191

Endotoxin

Less than 1.0 EU per μg by the LAL method.

Purity

>90% as determined by SDS-PAGE.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

SDS-PAGE

Bioactivity-ELISA 1

Immobilized Human VEGF R1&VEGF R2, Fc Tag at 2 μg/mL (100 μL/well) can bind Human VEGF165, His Tag with a linear range of 0.004-0.063 μg/mL.

Bioactivity-BLI 1

Loaded Avastin (Bevacizumab) on AHC Biosensor, can bind Human VEGF165, His Tag with an affinity constant of 0.448 nM as determined in BLI assay.

Bioactivity-SPR 1

Avastin (Bevacizumab) captured on CM5 chip via anti-human IgG Fc antibodies surface, can bind Human VEGF165 Protein, His Tag with an affinity constant of 1.03 nM as determined in a SPR assay.

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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