Recombinant Human VEGFA(VEGF165) protein, N-His Tag

Product Description

Cat

IMP-621

Official Symbol

VEGFA

Product Overview

Recombinant Human VEGFA protein(NP_001020537.2)(Ala 27-Arg 191), fused to N-His Tag, was expressed in HEK293.

Expression System

HEK293

Species

Human

Tag

N-His Tag

Form

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Molecular Mass

The protein has a calculated MW of 21.1 kDa. The protein migrates as 25-27 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Protein length

Ala 27-Arg 191

Endotoxin

Less than 1.0 EU per μg by the LAL method.

Purity

>90% as determined by SDS-PAGE.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

SDS-PAGE

Bioactivity-ELISA 1

Immobilized Human VEGF R1&VEGF R2, Fc Tag at 2 μg/mL (100 μL/well) can bind Human VEGF165, His Tag with a linear range of 0.004-0.063 μg/mL.

Bioactivity-BLI 1

Loaded Avastin (Bevacizumab) on AHC Biosensor, can bind Human VEGF165, His Tag with an affinity constant of 0.448 nM as determined in BLI assay.

Bioactivity-SPR 1

Avastin (Bevacizumab) captured on CM5 chip via anti-human IgG Fc antibodies surface, can bind Human VEGF165 Protein, His Tag with an affinity constant of 1.03 nM as determined in a SPR assay.

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

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