Recombinant Human VEGFC Protein (Ala112-Arg227)
Product Description
Cat
IMP-9950
Official Symbol
VEGFC
Product Overview
Recombinant human VEGF-C protein (Ala112-Arg227) without tag was expressed in Chinese Hamster Ovary cell line.
Description
The protein encoded by this gene is a member of the platelet-derived growth factor/vascular endothelial growth factor (PDGF/VEGF) family. The encoded protein promotes angiogenesis and endothelial cell growth, and can also affect the permeability of blood vessels. The proprotein is further cleaved into a fully processed form that can bind and activate VEGFR-2 and VEGFR-3 receptors.
Expression System
CHO
Species
Human
Tag
Tag Free
Predicted N Terminal
Ala112
Form
Lyophilized from a 0.2 μm filtered solution in HCl with BSA as a carrier protein.
Molecular Mass
Predicted Molecular Mass: 13 kDa
SDS-PAGE: 13-20 kDa, reducing conditions
Protein length
Ala112-Arg227
Bio-activity
Measured in a cell proliferation assay using HMVEC human microvascular endothelial cells. Marconcini, L. et al. (1999) Proc. Natl. Acad. Sci. USA 96:9671. The ED50 for this effect is 1.5-9 ng/mL.
Endotoxin
<0.10 EU/μg of the protein by the LAL method.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.
Notes
Disulfide-linked homodimer
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 250 μg/mL in 4 mM HCl.
Bioactivity-ELISA 1
Bioactivity

Measured in a cell proliferation assay using HMVEC human microvascular endothelial cells. Marconcini, L. et al. (1999) Proc. Natl. Acad. Sci. USA 96:9671. The ED50 for this effect is 1.5-9 ng/mL.

Data Sheet MSDS
For research or industrial raw materials, not for personal medical use!

Online Inquiry
Contact Info