Recombinant Human VEGFC Protein (Ala112-Arg227)

Product Description

Cat

IMP-9950

Official Symbol

VEGFC

Product Overview

Recombinant human VEGF-C protein (Ala112-Arg227) without tag was expressed in Chinese Hamster Ovary cell line.

Description

The protein encoded by this gene is a member of the platelet-derived growth factor/vascular endothelial growth factor (PDGF/VEGF) family. The encoded protein promotes angiogenesis and endothelial cell growth, and can also affect the permeability of blood vessels. The proprotein is further cleaved into a fully processed form that can bind and activate VEGFR-2 and VEGFR-3 receptors.

Expression System

CHO

Species

Human

Tag

Tag Free

Predicted N Terminal

Ala112

Form

Lyophilized from a 0.2 μm filtered solution in HCl with BSA as a carrier protein.

Molecular Mass

Predicted Molecular Mass: 13 kDa
SDS-PAGE: 13-20 kDa, reducing conditions

Protein length

Ala112-Arg227

Bio-activity

Measured in a cell proliferation assay using HMVEC human microvascular endothelial cells. Marconcini, L. et al. (1999) Proc. Natl. Acad. Sci. USA 96:9671. The ED50 for this effect is 1.5-9 ng/mL.

Endotoxin

<0.10 EU/μg of the protein by the LAL method.

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.

Notes

Disulfide-linked homodimer

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.

Reconstitution

Reconstitute at 250 μg/mL in 4 mM HCl.

Bioactivity-ELISA 1

Measured in a cell proliferation assay using HMVEC human microvascular endothelial cells. Marconcini, L. et al. (1999) Proc. Natl. Acad. Sci. USA 96:9671. The ED50 for this effect is 1.5-9 ng/mL.

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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