Recombinant Human ZNRF3 protein, C-His Tag

Product Description

Cat

IMP-6067

Official Symbol

ZNRF3

Product Overview

Recombinant Human ZNRF3 protein(Q9ULT6)(56-219aa), fused with C-terminal His tag, was expressed in HEK293.

Expression System

HEK293

Species

Human

Tag

C-His Tag

Form

Lyophilized from a 0.2 μm filtered PBS, 6% Trehalose, pH 7.4

Molecular Mass

20.4 kDa

Protein length

56-219aa

AA Sequence

KETAFVEVVLFESSPSGDYTTYTTGLTGRFSRAGATLSAEGEIVQMHPLGLCNNNDEEDLYEYGWVGVVKLEQPELDPKPCLTVLGKAKRAVQRGATAVIFDVSENPEAIDQLNQGSEDPLKRPVVYVKGADAIKLMNIVNKQKVARARIQHRPPRQPTEYFDM

Endotoxin

Less than 1.0 EU/ug as determined by LAL method.

Purity

Greater than 95% as determined by SDS-PAGE.

Biological Activity

Measured by its binding ability in a functional ELISA. Immobilized Human ZNRF3 at 5 μg/mL can bind Human RSPO2, the EC50 is 5.091-6.991 ng/mL.

Storage

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

SDS-PAGE

Bioactivity-ELISA 1

Measured by its binding ability in a functional ELISA. Immobilized Human ZNRF3 at 5 ug/ml can bind Human RSPO2, the EC50 is 5.091-6.991 ng/mL

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

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