Recombinant Human ZNRF3 Protein (Lys56-Met219), C-hFc-tagged
Product Description
Cat
IMP-9906
Official Symbol
ZNRF3
Product Overview
Recombinant human ZNRF3 protein (Lys56-Met219) with a Human IgG1 (Pro100-Lys330) Fc tag at C-terminus was expressed in Chinese Hamster Ovary cell line.
Description
Enables frizzled binding activity and ubiquitin-protein transferase activity. Involved in cellular protein metabolic process and negative regulation of Wnt signaling pathway. Is integral component of plasma membrane.
Expression System
CHO
Species
Human
Tag
C-hFc
Predicted N Terminal
Lys56
Form
Lyophilized from a 0.2 μm filtered solution in PBS.
Molecular Mass
Predicted Molecular Mass: 44.8 kDa (monomer)
SDS-PAGE: 50-55 kDa, reducing conditions
Protein length
Lys56-Met219
Bio-activity
Measured by its binding ability in a functional ELISA. When Recombinant Human R-Spondin 3 is coated at 0.25 μg/mL, Recombinant Human ZNRF3 Fc Chimera binds with an apparent KD <3 nM.
Endotoxin
<0.01 EU/μg of the protein by the LAL method.
Purity
>95%, by SDS-PAGE with silver staining
Notes
Disulfide-linked homodimer
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 500 μg/mL in PBS.
Data Sheet MSDS
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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