Recombinant Human ZNRF3 Protein (Lys56-Met219), C-hFc-tagged
Product Description
Cat
IMP-9906
Official Symbol
ZNRF3
Product Overview
Recombinant human ZNRF3 protein (Lys56-Met219) with a Human IgG1 (Pro100-Lys330) Fc tag at C-terminus was expressed in Chinese Hamster Ovary cell line.
Description
Enables frizzled binding activity and ubiquitin-protein transferase activity. Involved in cellular protein metabolic process and negative regulation of Wnt signaling pathway. Is integral component of plasma membrane.
Expression System
CHO
Species
Human
Tag
C-hFc
Predicted N Terminal
Lys56
Form
Lyophilized from a 0.2 μm filtered solution in PBS.
Molecular Mass
Predicted Molecular Mass: 44.8 kDa (monomer)
SDS-PAGE: 50-55 kDa, reducing conditions
Protein length
Lys56-Met219
Bio-activity
Measured by its binding ability in a functional ELISA. When Recombinant Human R-Spondin 3 is coated at 0.25 μg/mL, Recombinant Human ZNRF3 Fc Chimera binds with an apparent KD <3 nM.
Endotoxin
<0.01 EU/μg of the protein by the LAL method.
Purity
>95%, by SDS-PAGE with silver staining
Notes
Disulfide-linked homodimer
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 500 μg/mL in PBS.
Data Sheet MSDS
Datasheet MSDS
For research or industrial raw materials, not for personal medical use!

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