Recombinant Human/Cynomolgus/Rhesus macaque CD28 protein, C-Fc Tag

Product Description

Cat

IMP-131

Official Symbol

CD28

Product Overview

Recombinant Human/Cynomolgus/Rhesus macaque CD28 protein(AA Asn 19-Pro 152(Accession # P10747-1)) is expressed from human 293 cells (HEK293). In the region Asn 19-Pro 152, the AA sequence of Human, Cynomolgus and Rhesus macaque CD28 are homologus. This protein carries a human IgG1 Fc tag at the C-terminus.

Expression System

HEK293

Species

Human/Cynomolgus/Rhesus macaque

Tag

C-Fc Tag

Form

Lyophilized from sterile Tris with Glycine, Arginine and NaCl, pH7.5, 10% trehalose.

Molecular Mass

This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 41.6 kDa. The protein migrates as 55-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Protein length

Asn 19-Pro 152

Endotoxin

Less than 1.0 EU per ug by the LAL method.

Purity

>95% as determined by SDS-PAGE.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4ºC before opening to recover the entire contents.

SDS-PAGE

Human / Cynomolgus / Rhesus macaque CD28, Fc Tag (HPLC-verified) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS 1

The purity of Human / Cynomolgus / Rhesus macaque CD28, Fc Tag (HPLC-verified) was greater than 90% as determined by SEC-HPLC.

Bioactivity-ELISA 1

Immobilized Human / Cynomolgus / Rhesus macaque CD28, Fc Tag (HPLC-verified) at 5 ug/mL (100 uL/well) can bind Biotinylated Human B7-2, Fc Tag with a linear range of 0.019-0.625 ug/mL (QC tested).

Bioactivity-ELISA 2

Immobilized Human / Cynomolgus / Rhesus macaque CD28, Fc Tag (HPLC-verified) at 2 ug/mL (100 uL/well) can bind Anti-Mouse CD28 MAb with a linear range of 0.1-2 ng/ml (QC tested).

Bioactivity-ELISA 3

Immobilized Human / Cynomolgus / Rhesus macaque CD28, Fc Tag (HPLC-verified) at 5 ug/mL (100 uL/well) can bind Biotinylated Human B7-1, Fc Tag with a linear range of 0.313-5 ug/mL (Routinely tested).

Bioactivity-BLI 1

Loaded Human / Cynomolgus / Rhesus macaque CD28, Fc Tag (HPLC-verified) on ProteinA Biosensor, can bind Human B7-1, His Tag with an affinity constant of 2.2 uM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Bioactivity-BLI 2

Loaded Human / Cynomolgus / Rhesus macaque CD28, Fc Tag (HPLC-verified) on Protein A Biosensor, can bind Human B7-2, His Tag with an affinity constant of 6.8 uM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Bioactivity-SPR 1

Immobilized Human / Cynomolgus / Rhesus macaque CD28, Fc Tag (HPLC-verified) on CM5 Chip can bind Human B7-1, His Tag with an affinity constant of 5.29 uM as determined in a SPR assay (Biacore T200) (Routinely tested).

Bioactivity-SPR 2

Immobilized Human / Cynomolgus / Rhesus macaque CD28, Fc Tag (HPLC-verified) on CM5 Chip can bind Human B7-1, Mouse IgG2a Fc Tag, low endotoxin with an affinity constant of 2.72 uM as determined in a SPR assay (Biacore T200) (Routinely tested).

Bioactivity-SPR 3

Immobilized Human / Cynomolgus / Rhesus macaque CD28, Fc Tag (HPLC-verified) on CM5 Chip can bind Human B7-1, Fc Tag (HPLC-verified) with an affinity constant of 2.01 uM as determined in a SPR assay (Biacore T200) (Routinely tested).

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

Online Inquiry