Recombinant Human/Cynomolgus/Rhesus macaque CD28 protein, C-Fc Tag

Product Description

Cat

IMP-131

Official Symbol

CD28

Product Overview

Recombinant Human/Cynomolgus/Rhesus macaque CD28 protein(AA Asn 19-Pro 152(Accession # P10747-1)) is expressed from human 293 cells (HEK293). In the region Asn 19-Pro 152, the AA sequence of Human, Cynomolgus and Rhesus macaque CD28 are homologus. This protein carries a human IgG1 Fc tag at the C-terminus.

Expression System

HEK293

Species

Human/Cynomolgus/Rhesus macaque

Tag

C-Fc Tag

Form

Lyophilized from sterile Tris with Glycine, Arginine and NaCl, pH7.5, 10% trehalose.

Molecular Mass

This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 41.6 kDa. The protein migrates as 55-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Protein length

Asn 19-Pro 152

Endotoxin

Less than 1.0 EU per ug by the LAL method.

Purity

>95% as determined by SDS-PAGE.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4ºC before opening to recover the entire contents.

SDS-PAGE

Human / Cynomolgus / Rhesus macaque CD28, Fc Tag (HPLC-verified) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS 1

The purity of Human / Cynomolgus / Rhesus macaque CD28, Fc Tag (HPLC-verified) was greater than 90% as determined by SEC-HPLC.

Bioactivity-ELISA 1

Immobilized Human / Cynomolgus / Rhesus macaque CD28, Fc Tag (HPLC-verified) at 5 ug/mL (100 uL/well) can bind Biotinylated Human B7-2, Fc Tag with a linear range of 0.019-0.625 ug/mL (QC tested).

Bioactivity-ELISA 2

Immobilized Human / Cynomolgus / Rhesus macaque CD28, Fc Tag (HPLC-verified) at 2 ug/mL (100 uL/well) can bind Anti-Mouse CD28 MAb with a linear range of 0.1-2 ng/ml (QC tested).

Bioactivity-ELISA 3

Immobilized Human / Cynomolgus / Rhesus macaque CD28, Fc Tag (HPLC-verified) at 5 ug/mL (100 uL/well) can bind Biotinylated Human B7-1, Fc Tag with a linear range of 0.313-5 ug/mL (Routinely tested).

Bioactivity-BLI 1

Loaded Human / Cynomolgus / Rhesus macaque CD28, Fc Tag (HPLC-verified) on ProteinA Biosensor, can bind Human B7-1, His Tag with an affinity constant of 2.2 uM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Bioactivity-BLI 2

Loaded Human / Cynomolgus / Rhesus macaque CD28, Fc Tag (HPLC-verified) on Protein A Biosensor, can bind Human B7-2, His Tag with an affinity constant of 6.8 uM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Bioactivity-SPR 1

Immobilized Human / Cynomolgus / Rhesus macaque CD28, Fc Tag (HPLC-verified) on CM5 Chip can bind Human B7-1, His Tag with an affinity constant of 5.29 uM as determined in a SPR assay (Biacore T200) (Routinely tested).

Bioactivity-SPR 2

Immobilized Human / Cynomolgus / Rhesus macaque CD28, Fc Tag (HPLC-verified) on CM5 Chip can bind Human B7-1, Mouse IgG2a Fc Tag, low endotoxin with an affinity constant of 2.72 uM as determined in a SPR assay (Biacore T200) (Routinely tested).

Bioactivity-SPR 3

Immobilized Human / Cynomolgus / Rhesus macaque CD28, Fc Tag (HPLC-verified) on CM5 Chip can bind Human B7-1, Fc Tag (HPLC-verified) with an affinity constant of 2.01 uM as determined in a SPR assay (Biacore T200) (Routinely tested).

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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