Recombinant Human/Monkey CD28 Protein (Asn19-Pro152), C-hFc and Avi-tagged, Biotinylated
Product Description
Cat
IMP-10663
Official Symbol
CD28
Product Overview
Biotinylated recombinant Human/Cynomolgus/Rhesus CD28 protein (Asn19-Pro152) with a Human IgG1 (Pro100-Lys330) Fc tag and Avi-tag at C-terminus was expressed in Chinese Hamster Ovary cell line.
Description
The protein encoded by this gene is essential for T-cell proliferation and survival, cytokine production, and T-helper type-2 development. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Expression System
CHO
Species
Human/Monkey
Tag
C-hFc and Avi
Predicted N Terminal
Asn19
Form
Supplied as a 0.2 μm filtered solution in PBS.
Conjugate
Biotinylated
Molecular Mass
Predicted Molecular Mass: 42 kDa (monomer)
SDS-PAGE: 120-140 kDa, under non-reducing conditions
Protein length
Asn19-Pro152
Bio-activity
Measured by its binding ability in a functional ELISA. When Recombinant Human B7-1/CD80 Fc Chimera Protein is immobilized at 10 μg/mL (100 μL/well), the concentration of Biotinylated Recombinant Cynomolgus Monkey/Rhesus Macaque CD28 Fc Chimera Avi-tag that produces a 50% optimal binding response is found to be 0.5-3 μg/mL.
Endotoxin
<0.10 EU/μg of the protein by the LAL method.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.
Notes
Disulfide-linked homodimer, biotinylated via Avi-tag
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
2 weeks, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Bioactivity-ELISA 1
Binding Activity

Measured by its binding ability in a functional ELISA. When Recombinant Human B7-1/CD80 Fc Chimera Protein is immobilized at 10 μg/mL (100 μL/well), the concentration of Biotinylated Recombinant Cynomolgus Monkey/Rhesus Macaque CD28 Fc Chimera Avi-tag that produces a 50% optimal binding response is found to be 0.5-3 μg/mL.

Data Sheet MSDS
For research or industrial raw materials, not for personal medical use!

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