Recombinant Human/Monkey CD28 Protein (Asn19-Pro152), C-hFc and Avi-tagged, Biotinylated
Product Description
Cat
IMP-10663
Official Symbol
CD28
Product Overview
Biotinylated recombinant Human/Cynomolgus/Rhesus CD28 protein (Asn19-Pro152) with a Human IgG1 (Pro100-Lys330) Fc tag and Avi-tag at C-terminus was expressed in Chinese Hamster Ovary cell line.
Description
The protein encoded by this gene is essential for T-cell proliferation and survival, cytokine production, and T-helper type-2 development. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Expression System
CHO
Species
Human/Monkey
Tag
C-hFc and Avi
Predicted N Terminal
Asn19
Form
Supplied as a 0.2 μm filtered solution in PBS.
Conjugate
Biotinylated
Molecular Mass
Predicted Molecular Mass: 42 kDa (monomer)
SDS-PAGE: 120-140 kDa, under non-reducing conditions
Protein length
Asn19-Pro152
Bio-activity
Measured by its binding ability in a functional ELISA. When Recombinant Human B7-1/CD80 Fc Chimera Protein is immobilized at 10 μg/mL (100 μL/well), the concentration of Biotinylated Recombinant Cynomolgus Monkey/Rhesus Macaque CD28 Fc Chimera Avi-tag that produces a 50% optimal binding response is found to be 0.5-3 μg/mL.
Endotoxin
<0.10 EU/μg of the protein by the LAL method.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.
Notes
Disulfide-linked homodimer, biotinylated via Avi-tag
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
2 weeks, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Bioactivity-ELISA 1
Binding Activity

Measured by its binding ability in a functional ELISA. When Recombinant Human B7-1/CD80 Fc Chimera Protein is immobilized at 10 μg/mL (100 μL/well), the concentration of Biotinylated Recombinant Cynomolgus Monkey/Rhesus Macaque CD28 Fc Chimera Avi-tag that produces a 50% optimal binding response is found to be 0.5-3 μg/mL.

Data Sheet MSDS
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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