Recombinant Human/Monkey CD28 Protein (Asn19-Pro152), C-hFc-tagged
Product Description
Cat
IMP-10383
Official Symbol
CD28
Product Overview
Recombinant Human/Cynomolgus/Rhesus Macaque CD28 (Asn19-Pro152) protein with a Human IgG1 (Pro100-Lys330) Fc tag at C-terminus was expressed in Mouse myeloma cell line.
Description
The protein encoded by this gene is essential for T-cell proliferation and survival, cytokine production, and T-helper type-2 development. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Expression System
Mouse myeloma cell line
Species
Human/Monkey
Tag
C-hFc
Predicted N Terminal
Asn19
Form
Lyophilized from a 0.2 μm filtered solution in PBS.
Molecular Mass
Predicted Molecular Mass: 42.2 kDa (monomer)
SDS-PAGE: 60-70 kDa, reducing conditions
Protein length
Asn19-Pro152
Bio-activity
Measured by its binding ability in a functional ELISA. When Recombinant Human/Cynomolgus Monkey/Rhesus Macaque CD28 Fc Chimera is coated at 1 μg/mL (100 μL/well), the concentration of Recombinant Human B7-1/CD80 Fc Chimera that produces 50% of the optimal binding response is approximately 0.05-0.3 μg/mL.
Endotoxin
<0.10 EU/μg of the protein by the LAL method.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.
Notes
Disulfide-linked homodimer
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 100 μg/mL in sterile PBS.
Data Sheet MSDS
For research or industrial raw materials, not for personal medical use!

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