Recombinant Human/Monkey CD28 Protein (Asn19-Pro152), C-hFc-tagged

Product Description

Cat

IMP-10383

Official Symbol

CD28

Product Overview

Recombinant Human/Cynomolgus/Rhesus Macaque CD28 (Asn19-Pro152) protein with a Human IgG1 (Pro100-Lys330) Fc tag at C-terminus was expressed in Mouse myeloma cell line.

Description

The protein encoded by this gene is essential for T-cell proliferation and survival, cytokine production, and T-helper type-2 development. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Expression System

Mouse myeloma cell line

Species

Human/Monkey

Tag

C-hFc

Predicted N Terminal

Asn19

Form

Lyophilized from a 0.2 μm filtered solution in PBS.

Molecular Mass

Predicted Molecular Mass: 42.2 kDa (monomer)
SDS-PAGE: 60-70 kDa, reducing conditions

Protein length

Asn19-Pro152

Bio-activity

Measured by its binding ability in a functional ELISA. When Recombinant Human/Cynomolgus Monkey/Rhesus Macaque CD28 Fc Chimera is coated at 1 μg/mL (100 μL/well), the concentration of Recombinant Human B7-1/CD80 Fc Chimera that produces 50% of the optimal binding response is approximately 0.05-0.3 μg/mL.

Endotoxin

<0.10 EU/μg of the protein by the LAL method.

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.

Notes

Disulfide-linked homodimer

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.

Reconstitution

Reconstitute at 100 μg/mL in sterile PBS.

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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