Recombinant Human/Mouse IL23(IL23A&IL12B) protein, C-His tag
Product Description
Cat
IMP-743
Official Symbol
IL23(IL23A&IL12B)
Product Overview
Recombinant Human/Mouse IL23(IL23A(Q9NPF7)(Arg20-Pro189) & IL12B(P43432)(Met23-Ser335)) protein, fused with a 6*His tag at the C-terminus, was expressed from HEK293 Cells.
Expression System
HEK293
Species
Human/Mouse
Tag
C-His tag
Form
Lyophilized from sterile PBS, pH 7.4.
Molecular Mass
The protein has a calculated MW of 56.9kDa, and migrates as 65-80kDa under reducing conditions.
Protein length
Arg20-Pro189&Met23-Ser335
Endotoxin
Less than 1.0 EU per ug as determined by the LAL method.
Purity
>95% as determined by reducing SDS-PAGE.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
Reconstitution
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4ºC before opening to recover the entire contents.
SDS-PAGE
SDS-PAGE

Bioactivity-ELISA 1
Bioactivity-Elisa

Immobilized Human IL-23R-Fc at 10μg/ml (100 μl/well) can bind Human IL-23A & mouse IL-12B Heterodimer-His. The ED50 of Human IL-23A & mouse IL-12B Heterodimer-His is 0.48 ug/ml.

Bioactivity-BLI 1

Loaded Recombinant Human IL-23R-Fc on Protein A Biosensor, can bind Recombinant Human IL-23A & mouse IL-12B Heterodimer-His with an affinity constant of 9.48 nM as determined in BLI assay.

Data Sheet MSDS
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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