Recombinant Human/Mouse IL23(IL23A&IL12B) protein, C-His tag

Product Description

Cat

IMP-743

Official Symbol

IL23(IL23A&IL12B)

Product Overview

Recombinant Human/Mouse IL23(IL23A(Q9NPF7)(Arg20-Pro189) & IL12B(P43432)(Met23-Ser335)) protein, fused with a 6*His tag at the C-terminus, was expressed from HEK293 Cells.

Expression System

HEK293

Species

Human/Mouse

Tag

C-His tag

Form

Lyophilized from sterile PBS, pH 7.4.

Molecular Mass

The protein has a calculated MW of 56.9kDa, and migrates as 65-80kDa under reducing conditions.

Protein length

Arg20-Pro189&Met23-Ser335

Endotoxin

Less than 1.0 EU per ug as determined by the LAL method.

Purity

>95% as determined by reducing SDS-PAGE.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4ºC before opening to recover the entire contents.

SDS-PAGE

Bioactivity-ELISA 1

Immobilized Human IL-23R-Fc at 10μg/ml (100 μl/well) can bind Human IL-23A & mouse IL-12B Heterodimer-His. The ED50 of Human IL-23A & mouse IL-12B Heterodimer-His is 0.48 ug/ml.

Bioactivity-BLI 1

Loaded Recombinant Human IL-23R-Fc on Protein A Biosensor, can bind Recombinant Human IL-23A & mouse IL-12B Heterodimer-His with an affinity constant of 9.48 nM as determined in BLI assay.

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

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