Recombinant Marmoset IL23A Protein, C-His-tagged
Product Description
Cat
IMP-4700
Official Symbol
IL23A
Product Overview
Recombinant marmoset IL23A (Met 1-Pro 189) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 1; Recombinant marmoset IL12B (Met 1-Asn328) was expressed with a polyhistidine tag at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the marmoset IL23A&the marmoset IL12B heterodimer was purified.
Description
Associates with IL12B to form the IL-23 interleukin, an heterodimeric cytokine which functions in innate and adaptive immunity. IL-23 may constitute with IL-17 an acute response to infection in peripheral tissues. IL-23 binds to an heterodimeric receptor complex composed of IL12RB1 and IL23R, activates the Jak-Stat signaling cascade, stimulates memory rather than naive T-cells and promotes production of proinflammatory cytokines. IL-23 induces autoimmune inflammation and thus may be responsible for autoimmune inflammatory diseases and may be important for tumorigenesis.
Expression System
HEK293
Species
Marmoset
Tag
C-His
Predicted N Terminal
Arg 20&Ile 23
Form
Lyophilized from sterile PBS, pH 7.4, 5 % trehalose, 5% mannitol and 0.01% Tween80.
Molecular Mass
The recombinant marmoset IL23A&the marmoset IL12B comprises 498(181+317) amino acids and has a calculated molecular mass of 56.4 (20.1+36.3) KDa. The apparent molecular mass of the marmoset IL23A&the marmoset IL12B heterodimer is approximately 22&45 KDa respectively in SDS-PAGE.
Protein length
Met1-Asn328
Endotoxin
< 1.0 EU/μg of the protein as determined by the LAL method
Purity
> 85 % as determined by SDS-PAGE
Storage
Samples are stable for up to twelve months from date of receipt at -20 to -80 centigrade.
Store it under sterile conditions at -20 to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
SDS-PAGE
SDS-PAGE

Data Sheet MSDS
For research or industrial raw materials, not for personal medical use!

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