Recombinant Monkey CD2 Protein (Lys25-Asp209), C-hFc-tagged
Product Description
Cat
IMP-10252
Official Symbol
CD2
Product Overview
Recombinant Cynomolgus CD2 Fc Chimera protein (Lys25-Asp209) with a Human IgG1 (Pro100-Lys330) Fc tag at C-terminus was expressed in Chinese Hamster Ovary cell line.
Description
The protein encoded by this gene is a surface antigen found on all peripheral blood T-cells. The encoded protein interacts with LFA3 (CD58) on antigen presenting cells to optimize immune recognition. A locus control region (LCR) has been found in the 3' flanking sequence of this gene.
Expression System
CHO
Species
Monkey
Tag
C-hFc
Predicted N Terminal
Lys25
Form
Lyophilized from a 0.2 μm filtered solution in PBS.
Molecular Mass
Predicted Molecular Mass: 48 kDa
SDS-PAGE: 60-66 kDa, under reducing conditions
Protein length
Lys25-Asp209
Bio-activity
Measured by its binding ability in a functional ELISA. When Recombinant Cynomolgus Monkey CD2 Fc Chimera is immobilized at 0.25 μg/mL (100 μL/well), the concentration of Recombinant Human CD58/LFA-3 Fc Chimera that produces 50% of the optimal binding response is 0.15-0.9 μg/mL.
Endotoxin
<0.10 EU/μg of the protein by the LAL method.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.
Notes
Disulfide-linked homodimer
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 100 μg/mL in PBS.
SDS-PAGE
SDS-PAGE

Bioactivity-ELISA 1
Binding Activity

Measured by its binding ability in a functional ELISA. When Recombinant Cynomolgus Monkey CD2 Fc Chimera is immobilized at 0.25 μg/mL (100 μL/well), the concentration of Recombinant Human CD58/LFA-3 Fc Chimera that produces 50% of the optimal binding response is 0.15-0.9 μg/mL.

Data Sheet MSDS
Datasheet MSDS
For research or industrial raw materials, not for personal medical use!

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    1-516-512-3133
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