Recombinant Monkey CD2 Protein (Lys25-Asp209), C-hFc-tagged

Product Description

Cat

IMP-10252

Official Symbol

CD2

Product Overview

Recombinant Cynomolgus CD2 Fc Chimera protein (Lys25-Asp209) with a Human IgG1 (Pro100-Lys330) Fc tag at C-terminus was expressed in Chinese Hamster Ovary cell line.

Description

The protein encoded by this gene is a surface antigen found on all peripheral blood T-cells. The encoded protein interacts with LFA3 (CD58) on antigen presenting cells to optimize immune recognition. A locus control region (LCR) has been found in the 3' flanking sequence of this gene.

Expression System

CHO

Species

Monkey

Tag

C-hFc

Predicted N Terminal

Lys25

Form

Lyophilized from a 0.2 μm filtered solution in PBS.

Molecular Mass

Predicted Molecular Mass: 48 kDa
SDS-PAGE: 60-66 kDa, under reducing conditions

Protein length

Lys25-Asp209

Bio-activity

Measured by its binding ability in a functional ELISA. When Recombinant Cynomolgus Monkey CD2 Fc Chimera is immobilized at 0.25 μg/mL (100 μL/well), the concentration of Recombinant Human CD58/LFA-3 Fc Chimera that produces 50% of the optimal binding response is 0.15-0.9 μg/mL.

Endotoxin

<0.10 EU/μg of the protein by the LAL method.

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.

Notes

Disulfide-linked homodimer

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.

Reconstitution

Reconstitute at 100 μg/mL in PBS.

SDS-PAGE

Bioactivity-ELISA 1

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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