Recombinant Monkey CD2 Protein (Lys25-Asp209), C-hFc-tagged

Product Description

Cat

IMP-10252

Official Symbol

CD2

Product Overview

Recombinant Cynomolgus CD2 Fc Chimera protein (Lys25-Asp209) with a Human IgG1 (Pro100-Lys330) Fc tag at C-terminus was expressed in Chinese Hamster Ovary cell line.

Description

The protein encoded by this gene is a surface antigen found on all peripheral blood T-cells. The encoded protein interacts with LFA3 (CD58) on antigen presenting cells to optimize immune recognition. A locus control region (LCR) has been found in the 3' flanking sequence of this gene.

Expression System

CHO

Species

Monkey

Tag

C-hFc

Predicted N Terminal

Lys25

Form

Lyophilized from a 0.2 μm filtered solution in PBS.

Molecular Mass

Predicted Molecular Mass: 48 kDa
SDS-PAGE: 60-66 kDa, under reducing conditions

Protein length

Lys25-Asp209

Bio-activity

Measured by its binding ability in a functional ELISA. When Recombinant Cynomolgus Monkey CD2 Fc Chimera is immobilized at 0.25 μg/mL (100 μL/well), the concentration of Recombinant Human CD58/LFA-3 Fc Chimera that produces 50% of the optimal binding response is 0.15-0.9 μg/mL.

Endotoxin

<0.10 EU/μg of the protein by the LAL method.

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.

Notes

Disulfide-linked homodimer

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.

Reconstitution

Reconstitute at 100 μg/mL in PBS.

SDS-PAGE

Bioactivity-ELISA 1

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

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