Recombinant Monkey CD27 Protein (Ala20-Arg191), C-hFc-tagged
Product Description
Cat
IMP-10287
Official Symbol
CD27
Product Overview
Recombinant Cynomolgus/Rhesus Macaque CD27 protein (Ala20-Arg191) with a Human IgG1 (Pro100-Lys330) Fc tag at C-terminus was expressed in Chinese Hamster Ovary cell line.
Description
The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is required for generation and long-term maintenance of T cell immunity. It binds to ligand CD70, and plays a key role in regulating B-cell activation and immunoglobulin synthesis. This receptor transduces signals that lead to the activation of NF-kappaB and MAPK8/JNK. Adaptor proteins TRAF2 and TRAF5 have been shown to mediate the signaling process of this receptor. CD32-binding protein (SIVA), a proapoptotic protein, can bind to this receptor and is thought to play an important role in the apoptosis induced by this receptor.
Expression System
CHO
Species
Monkey
Tag
C-hFc
Predicted N Terminal
Thr21
Form
Lyophilized from a 0.2 μm filtered solution in PBS.
Molecular Mass
Predicted Molecular Mass: 46 kDa
SDS-PAGE: 61-68 kDa, reducing conditions
Protein length
Ala20-Arg191
Bio-activity
Measured by its ability to inhibit CD70-induced IL-8 secretion in HT1080 human fibrosarcoma cells transfected with human CD27. The ED50 for this effect is 0.25-1.5 μg/mL.
Endotoxin
<0.10 EU/μg of the protein by the LAL method.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.
Notes
Disulfide-linked homodimer
Storage
12 months from date of receipt, ≤ -20 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, ≤ -20 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 500 μg/mL in PBS.
SDS-PAGE
SDS-PAGE

Data Sheet MSDS
For research or industrial raw materials, not for personal medical use!

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