Recombinant Monkey CD33 Protein (Met16-Gly248), C-hFc-tagged
Product Description
Cat
IMP-10391
Official Symbol
CD33
Product Overview
Recombinant Cynomolgus/Rhesus Siglec-3/CD33 protein (Met16-Gly248) with a Human IgG1 (Pro100-Lys330) Fc tag at C-terminus was expressed in Chinese Hamster Ovary cell line.
Description
Enables protein phosphatase binding activity and sialic acid binding activity. Involved in several processes, including negative regulation of cytokine production; negative regulation of monocyte activation; and positive regulation of protein tyrosine phosphatase activity. Located in several cellular components, including Golgi apparatus; external side of plasma membrane; and peroxisome.
Expression System
CHO
Species
Monkey
Tag
C-hFc
Predicted N Terminal
Met16
Form
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Molecular Mass
Predicted Molecular Mass: 52 kDa
SDS-PAGE: 68-79 kDa, under reducing conditions
Protein length
Met16-Gly248
Bio-activity
Measured by its binding ability in a functional ELISA. When Recombinant Cynomolgus Monkey/Rhesus Macaque Siglec-3/CD33 Fc Chimera is immobilized at 1 μg/mL (100 μL/well), Recombinant Human Galectin-3BP/MAC-2BP binds with an ED50 of 0.15-1.20 μg/mL.
Endotoxin
<0.10 EU/μg of the protein by the LAL method.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.
Notes
Disulfide-linked homodimer
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 500 μg/mL in PBS.
SDS-PAGE
SDS-PAGE

Bioactivity-ELISA 1
Bioactivity

Measured by its binding ability in a functional ELISA. When Recombinant Cynomolgus Monkey/Rhesus Macaque Siglec-3/CD33 Fc Chimera is immobilized at 1 μg/mL (100 μL/well), Recombinant Human Galectin-3BP/MAC-2BP binds with an ED50 of 0.15-1.20 μg/mL.

Data Sheet MSDS
For research or industrial raw materials, not for personal medical use!

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