Recombinant Monkey CD48 Protein (Gln27-Ser217), C-6×His-tagged
Product Description
Cat
IMP-10344
Official Symbol
CD48
Product Overview
Recombinant Cynomolgus CD48/SLAMF2 protein (Gln27-Ser217) with a C-terminal 6×His tag was expressed in Chinese Hamster Ovary cell line.
Description
This gene encodes a member of the CD2 subfamily of immunoglobulin-like receptors which includes SLAM (signaling lymphocyte activation molecules) proteins. The encoded protein is found on the surface of lymphocytes and other immune cells, dendritic cells and endothelial cells, and participates in activation and differentiation pathways in these cells. The encoded protein does not have a transmembrane domain, however, but is held at the cell surface by a GPI anchor via a C-terminal domain which maybe cleaved to yield a soluble form of the receptor. Multiple transcript variants encoding different isoforms have been found for this gene.
Expression System
CHO
Species
Monkey
Tag
C-6×His
Predicted N Terminal
Gln27 inferred from enzymatic pyroglutamate treatment revealing Gly28
Form
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Molecular Mass
Predicted Molecular Mass: 23 kDa
SDS-PAGE: 39-44 kDa, under reducing conditions
Protein length
Gln27-Ser217
Bio-activity
Measured by its binding ability in a functional ELISA. When Recombinabt Human 2B4/CD244/SLAMF4 Fc Chimera is immobilized at 0.5 μg/mL, 100 μL/well, the concentration of Recombinant Cynomolgus Monkey CD48/SLAMF2 His-tag that produces 50% of the optimal binding response is approximately 75-450 ng/mL.
Endotoxin
<0.10 EU/μg of the protein by the LAL method.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 200 μg/mL in PBS.
SDS-PAGE
SDS-PAGE

Bioactivity-ELISA 1
Binding Activity

Measured by its binding ability in a functional ELISA. When Recombinabt Human 2B4/CD244/SLAMF4 Fc Chimera is immobilized at 0.5 μg/mL, 100 μL/well, the concentration of Recombinant Cynomolgus Monkey CD48/SLAMF2 His-tag that produces 50% of the optimal binding response is approximately 75-450 ng/mL.

Data Sheet MSDS
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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