Recombinant Monkey CD86 Protein (Leu20-His239), C-6×His-tagged
Product Description
Cat
IMP-10304
Official Symbol
CD86
Product Overview
Recombinant Cynomolgus Monkey B7-2/CD86 protein (Leu20-His239) with a C-terminal 6×His tag was expressed in Human embryonic kidney cell.
Description
This gene encodes a type I membrane protein that is a member of the immunoglobulin superfamily. This protein is expressed by antigen-presenting cells, and it is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of this protein with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of this protein with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response. Alternative splicing results in several transcript variants encoding different isoforms.
Expression System
HEK293
Species
Monkey
Tag
C-6×His
Predicted N Terminal
Leu20
Form
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Molecular Mass
Predicted Molecular Mass: 26 kDa
SDS-PAGE: 57-68 kDa, under reducing conditions
Protein length
Leu20-His239
Bio-activity
Measured by its binding ability in a functional ELISA. When Recombinant Cynomolgus Monkey B7-2/CD86 is immobilized at 0.5 μg/mL (100 μL/well), it binds to Recombinant Human CTLA-4 Fc Chimera with an ED50 of 5-25 ng/mL.
Endotoxin
<0.10 EU/μg of the protein by the LAL method.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 500 μg/mL in PBS.
SDS-PAGE
SDS-PAGE

Bioactivity-ELISA 1
Binding Activity

Measured by its binding ability in a functional ELISA. When Recombinant Cynomolgus Monkey B7-2/CD86 is immobilized at 0.5 μg/mL (100 μL/well), it binds to Recombinant Human CTLA-4 Fc Chimera with an ED50 of 5-25 ng/mL.

Data Sheet MSDS
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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