Recombinant Monkey CD86 Protein (Leu20-His239), C-6×His-tagged
Product Description
Cat
IMP-10304
Official Symbol
CD86
Product Overview
Recombinant Cynomolgus Monkey B7-2/CD86 protein (Leu20-His239) with a C-terminal 6×His tag was expressed in Human embryonic kidney cell.
Description
This gene encodes a type I membrane protein that is a member of the immunoglobulin superfamily. This protein is expressed by antigen-presenting cells, and it is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of this protein with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of this protein with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response. Alternative splicing results in several transcript variants encoding different isoforms.
Expression System
HEK293
Species
Monkey
Tag
C-6×His
Predicted N Terminal
Leu20
Form
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Molecular Mass
Predicted Molecular Mass: 26 kDa
SDS-PAGE: 57-68 kDa, under reducing conditions
Protein length
Leu20-His239
Bio-activity
Measured by its binding ability in a functional ELISA. When Recombinant Cynomolgus Monkey B7-2/CD86 is immobilized at 0.5 μg/mL (100 μL/well), it binds to Recombinant Human CTLA-4 Fc Chimera with an ED50 of 5-25 ng/mL.
Endotoxin
<0.10 EU/μg of the protein by the LAL method.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 500 μg/mL in PBS.
SDS-PAGE
SDS-PAGE

Bioactivity-ELISA 1
Binding Activity

Measured by its binding ability in a functional ELISA. When Recombinant Cynomolgus Monkey B7-2/CD86 is immobilized at 0.5 μg/mL (100 μL/well), it binds to Recombinant Human CTLA-4 Fc Chimera with an ED50 of 5-25 ng/mL.

Data Sheet MSDS
For research or industrial raw materials, not for personal medical use!

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