Recombinant Monkey EGFR Protein (Leu25-Ser645), C-6×His-tagged
Product Description
Cat
IMP-9586
Official Symbol
EGFR
Product Overview
Recombinant Cynomolgus Monkey EGFR protein (Leu25-Ser645) with a C-terminal 6×His tag was expressed in Chinese Hamster Ovary cell line.
Description
The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor, thus inducing receptor dimerization and tyrosine autophosphorylation leading to cell proliferation. Mutations in this gene are associated with lung cancer. EGFR is a component of the cytokine storm which contributes to a severe form of Coronavirus Disease 2019 (COVID-19) resulting from infection with severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2).
Expression System
CHO
Species
Monkey
Tag
C-6×His
Predicted N Terminal
Leu25
Form
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Molecular Mass
Predicted Molecular Mass: 69 kDa
SDS-PAGE: 95-109 kDa, under reducing conditions
Protein length
Leu25-Ser645
Bio-activity
Measured by its binding ability in a functional ELISA. When Recombinant Cynomolgus Monkey EGFR His-tag is immobilized at 20 μg/mL, 100 μL/well, the concentration of Recombinant Human Pro-EGF (aa 21-1023) Protein that produces 50% of the optimal binding response is approximately 100-600 ng/mL.
Endotoxin
<0.10 EU/μg of the protein by the LAL method.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 200 μg/mL in PBS.
SDS-PAGE
SDS-PAGE

Bioactivity-ELISA 1
Bioactivity

Measured by its binding ability in a functional ELISA. When Recombinant Cynomolgus Monkey EGFR His-tag is immobilized at 20 μg/mL, 100 μL/well, the concentration of Recombinant Human Pro-EGF (aa 21-1023) Protein that produces 50% of the optimal binding response is approximately 100-600 ng/mL.

Data Sheet MSDS
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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