Recombinant Monkey EPCAM Protein (Gln24-Lys265), C-6×His-tagged
Product Description
Cat
IMP-9595
Official Symbol
EPCAM
Product Overview
Recombinant Cynomolgus/Rhesus EpCAM/TROP1 protein (Gln24-Lys265), with a C-terminal 6×His tag was expressed in Chinese Hamster Ovary cell line.
Description
This gene encodes a carcinoma-associated antigen and is a member of a family that includes at least two type I membrane proteins. This antigen is expressed on most normal epithelial cells and gastrointestinal carcinomas and functions as a homotypic calcium-independent cell adhesion molecule. The antigen is being used as a target for immunotherapy treatment of human carcinomas. Mutations in this gene result in congenital tufting enteropathy.
Expression System
CHO
Species
Monkey
Tag
C-6×His
Predicted N Terminal
Gln24; inferred from deblocking reaction revealing Lys25
Form
Lyophilized from a 0.2 μm filtered solution in PBS.
Molecular Mass
Predicted Molecular Mass: 28 kDa
SDS-PAGE: 30-43 kDa, under reducing conditions.
Protein length
Gln24-Lys265
Bio-activity
Measured by the ability of the immobilized protein to support the adhesion of the L Cells mouse fibroblast cell line. The ED50 for this effect is 0.3-1.5 μg/mL.
Endotoxin
<0.10 EU/μg of the protein by the LAL method.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 500 μg/mL in PBS.
SDS-PAGE
SDS-PAGE

Bioactivity-ELISA 1
Bioactivity

Measured by the ability of the immobilized protein to support the adhesion of the L Cells mouse fibroblast cell line. The ED50 for this effect is 0.3-1.5 μg/mL.

Data Sheet MSDS
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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