Recombinant Monkey EPCAM Protein (Gln24-Lys265), C-6×His-tagged
Product Description
Cat
IMP-9595
Official Symbol
EPCAM
Product Overview
Recombinant Cynomolgus/Rhesus EpCAM/TROP1 protein (Gln24-Lys265), with a C-terminal 6×His tag was expressed in Chinese Hamster Ovary cell line.
Description
This gene encodes a carcinoma-associated antigen and is a member of a family that includes at least two type I membrane proteins. This antigen is expressed on most normal epithelial cells and gastrointestinal carcinomas and functions as a homotypic calcium-independent cell adhesion molecule. The antigen is being used as a target for immunotherapy treatment of human carcinomas. Mutations in this gene result in congenital tufting enteropathy.
Expression System
CHO
Species
Monkey
Tag
C-6×His
Predicted N Terminal
Gln24; inferred from deblocking reaction revealing Lys25
Form
Lyophilized from a 0.2 μm filtered solution in PBS.
Molecular Mass
Predicted Molecular Mass: 28 kDa
SDS-PAGE: 30-43 kDa, under reducing conditions.
Protein length
Gln24-Lys265
Bio-activity
Measured by the ability of the immobilized protein to support the adhesion of the L Cells mouse fibroblast cell line. The ED50 for this effect is 0.3-1.5 μg/mL.
Endotoxin
<0.10 EU/μg of the protein by the LAL method.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 500 μg/mL in PBS.
SDS-PAGE
SDS-PAGE

Bioactivity-ELISA 1
Bioactivity

Measured by the ability of the immobilized protein to support the adhesion of the L Cells mouse fibroblast cell line. The ED50 for this effect is 0.3-1.5 μg/mL.

Data Sheet MSDS
For research or industrial raw materials, not for personal medical use!

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