Recombinant Monkey FCGR2A Protein (Thr29-Ile211), C-6×His-tagged
Product Description
Cat
IMP-9955
Official Symbol
FCGR2A
Product Overview
Recombinant Cynomolgus Fc gamma RIIA/CD32a protein (Thr29-Ile211) with a C-terminal 6×His tag was expressed in human embryonic kidney cell.
Description
This gene encodes one member of a family of immunoglobulin Fc receptor genes found on the surface of many immune response cells. The protein encoded by this gene is a cell surface receptor found on phagocytic cells such as macrophages and neutrophils, and is involved in the process of phagocytosis and clearing of immune complexes. Alternative splicing results in multiple transcript variants.
Expression System
HEK293
Species
Monkey
Tag
C-6×His
Predicted N Terminal
Thr29
Form
Lyophilized from a 0.2 μm filtered solution in PBS.
Molecular Mass
Predicted Molecular Mass: 21 kDa
SDS-PAGE: 23-32 kDa, reducing conditions
Protein length
Thr29-Ile211
Bio-activity
Measured by its binding ability in a functional ELISA. When Recombinant Cynomolgus Monkey Fc gamma RIIA/CD32a is immobilized on a His Tag Antibody coated plate, it binds Biotinylated Human IgG. The concentration of Biotinylated Human IgG that produces 50% of the optimal binding response is approximately 0.75-3.75 μg/mL.
Endotoxin
<0.10 EU/μg of the protein by the LAL method.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 200 μg/mL in PBS.
Bioactivity-ELISA 1
Bioactivity

Measured by its binding ability in a functional ELISA. When Recombinant Cynomolgus Monkey Fc gamma RIIA/CD32a is immobilized on a His Tag Antibody coated plate, it binds Biotinylated Human IgG. The concentration of Biotinylated Human IgG that produces 50% of the optimal binding response is approximately 0.75-3.75 μg/mL.

Data Sheet MSDS
For research or industrial raw materials, not for personal medical use!

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