Recombinant Monkey HHLA2 Protein (Ile21-Asn345), C-6×His-tagged

Product Description

Cat

IMP-10408

Official Symbol

HHLA2

Product Overview

Recombinant Cynomolgus B7-H7/HHLA2 protein (Ile21-Asn345) with a C-terminal 6×His tag was expressed in Human embryonic kidney cell.

Description

This gene encodes a protein ligand found on the surface of monocytes. The encoded protein is thought to regulate cell-mediated immunity by binding to a receptor on T lymphocytes and inhibiting the proliferation of these cells. Alternate splicing results in multiple transcript variants.

Expression System

HEK293

Species

Monkey

Tag

C-6×His

Predicted N Terminal

Ile21

Form

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Molecular Mass

Predicted Molecular Mass: 38 kDa
SDS-PAGE: 62-70 kDa, reducing conditions

Protein length

Ile21-Asn345

Bio-activity

Measured by its binding ability in a functional ELISA. When Recombinant Human Human TMIGD2/CD28H Fc Chimera is immobilized at 1 μg/mL (100 μL/well), the concentration of Recombinant Cynomolgus Monkey B7-H7/HHLA2 that produces 50% of the optimal binding response is 0.2-1.2 μg/mL.

Endotoxin

<0.10 EU/μg of the protein by the LAL method.

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.

Reconstitution

Reconstitute at 500 μg/mL in PBS.

SDS-PAGE

Bioactivity-ELISA 1

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

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