Recombinant Monkey HHLA2 Protein (Ile21-Asn345), C-6×His-tagged

Product Description

Cat

IMP-10408

Official Symbol

HHLA2

Product Overview

Recombinant Cynomolgus B7-H7/HHLA2 protein (Ile21-Asn345) with a C-terminal 6×His tag was expressed in Human embryonic kidney cell.

Description

This gene encodes a protein ligand found on the surface of monocytes. The encoded protein is thought to regulate cell-mediated immunity by binding to a receptor on T lymphocytes and inhibiting the proliferation of these cells. Alternate splicing results in multiple transcript variants.

Expression System

HEK293

Species

Monkey

Tag

C-6×His

Predicted N Terminal

Ile21

Form

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Molecular Mass

Predicted Molecular Mass: 38 kDa
SDS-PAGE: 62-70 kDa, reducing conditions

Protein length

Ile21-Asn345

Bio-activity

Measured by its binding ability in a functional ELISA. When Recombinant Human Human TMIGD2/CD28H Fc Chimera is immobilized at 1 μg/mL (100 μL/well), the concentration of Recombinant Cynomolgus Monkey B7-H7/HHLA2 that produces 50% of the optimal binding response is 0.2-1.2 μg/mL.

Endotoxin

<0.10 EU/μg of the protein by the LAL method.

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.

Reconstitution

Reconstitute at 500 μg/mL in PBS.

SDS-PAGE

Bioactivity-ELISA 1

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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