Recombinant Monkey HHLA2 Protein (Ile21-Asn345), C-hFc-tagged

Product Description

Cat

IMP-10407

Official Symbol

HHLA2

Product Overview

Recombinant Cynomolgus B7-H7/HHLA2 protein (Ile21-Asn345) with a Human IgG1 (Pro100-Lys330) Fc tag at C-terminus was expressed in Human embryonic kidney cell.

Description

This gene encodes a protein ligand found on the surface of monocytes. The encoded protein is thought to regulate cell-mediated immunity by binding to a receptor on T lymphocytes and inhibiting the proliferation of these cells. Alternate splicing results in multiple transcript variants.

Expression System

HEK293

Species

Monkey

Tag

C-hFc

Predicted N Terminal

Ile21

Form

Lyophilized from a 0.2 μm filtered solution in Citric Acid and NaCl with Trehalose.

Molecular Mass

Predicted Molecular Mass: 64 kDa
SDS-PAGE: 92-109 kDa, reducing conditions

Protein length

Ile21-Asn345

Bio-activity

Measured by its ability to inhibit anti-CD3 antibody induced IL-2 secretion in human T lymphocytes. The ED50 for this effect is 1-6 μg/mL.

Endotoxin

<1.0 EU/μg of the protein by the LAL method.

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Notes

Disulfide-linked homodimer

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.

Reconstitution

Reconstitute at 200 μg/mL in water.

SDS-PAGE

Bioactivity-ELISA 1

Measured by its ability to inhibit anti-CD3 antibody induced IL-2 secretion in human T lymphocytes. The ED50 for this effect is 1-6 μg/mL.

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

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