Recombinant Monkey HHLA2 Protein (Ile21-Asn345), C-hFc-tagged

Product Description

Cat

IMP-10407

Official Symbol

HHLA2

Product Overview

Recombinant Cynomolgus B7-H7/HHLA2 protein (Ile21-Asn345) with a Human IgG1 (Pro100-Lys330) Fc tag at C-terminus was expressed in Human embryonic kidney cell.

Description

This gene encodes a protein ligand found on the surface of monocytes. The encoded protein is thought to regulate cell-mediated immunity by binding to a receptor on T lymphocytes and inhibiting the proliferation of these cells. Alternate splicing results in multiple transcript variants.

Expression System

HEK293

Species

Monkey

Tag

C-hFc

Predicted N Terminal

Ile21

Form

Lyophilized from a 0.2 μm filtered solution in Citric Acid and NaCl with Trehalose.

Molecular Mass

Predicted Molecular Mass: 64 kDa
SDS-PAGE: 92-109 kDa, reducing conditions

Protein length

Ile21-Asn345

Bio-activity

Measured by its ability to inhibit anti-CD3 antibody induced IL-2 secretion in human T lymphocytes. The ED50 for this effect is 1-6 μg/mL.

Endotoxin

<1.0 EU/μg of the protein by the LAL method.

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Notes

Disulfide-linked homodimer

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.

Reconstitution

Reconstitute at 200 μg/mL in water.

SDS-PAGE

Bioactivity-ELISA 1

Measured by its ability to inhibit anti-CD3 antibody induced IL-2 secretion in human T lymphocytes. The ED50 for this effect is 1-6 μg/mL.

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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