Recombinant Monkey ICOS Protein (Glu21-Gln134), C-hFc-tagged
Product Description
Cat
IMP-10414
Official Symbol
ICOS
Product Overview
Recombinant Cynomolgus ICOS protein (Glu21-Gln134) with a Human IgG1 (Pro100-Lys330) Fc tag at C-terminus was expressed in Chinese Hamster Ovary cell line.
Description
The protein encoded by this gene belongs to the CD28 and CTLA-4 cell-surface receptor family. It forms homodimers and plays an important role in cell-cell signaling, immune responses, and regulation of cell proliferation.
Expression System
CHO
Species
Monkey
Tag
C-hFc
Predicted N Terminal
Glu21
Form
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Molecular Mass
Predicted Molecular Mass: 40 kDa
SDS-PAGE: 40-61 kDa, reducing conditions
Protein length
Glu21-Gln134
Bio-activity
Measured by its binding ability in a functional ELISA. When Recombinant Cynomolgus Monkey B7-H2 is coated at 0.5 μg/mL (100 μL/well), the concentration of Recombinant Cynomolgus Monkey ICOS Fc Chimera that produces 50% optimal binding response is 0.75-3.75 ng/mL.
Endotoxin
<0.10 EU/μg of the protein by the LAL method.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.
Notes
Disulfide-linked homodimer
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 500 μg/mL in PBS.
Bioactivity-ELISA 1
Bioactivity

Measured by its binding ability in a functional ELISA. When Recombinant Cynomolgus Monkey B7-H2 is coated at 0.5 μg/mL (100 μL/well), the concentration of Recombinant Cynomolgus Monkey ICOS Fc Chimera that produces 50% optimal binding response is 0.75-3.75 ng/mL.

Data Sheet MSDS
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

Online Inquiry
Contact Info