Recombinant Monkey ICOS Protein (Glu21-Gln134), C-hFc-tagged
Product Description
Cat
IMP-10414
Official Symbol
ICOS
Product Overview
Recombinant Cynomolgus ICOS protein (Glu21-Gln134) with a Human IgG1 (Pro100-Lys330) Fc tag at C-terminus was expressed in Chinese Hamster Ovary cell line.
Description
The protein encoded by this gene belongs to the CD28 and CTLA-4 cell-surface receptor family. It forms homodimers and plays an important role in cell-cell signaling, immune responses, and regulation of cell proliferation.
Expression System
CHO
Species
Monkey
Tag
C-hFc
Predicted N Terminal
Glu21
Form
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Molecular Mass
Predicted Molecular Mass: 40 kDa
SDS-PAGE: 40-61 kDa, reducing conditions
Protein length
Glu21-Gln134
Bio-activity
Measured by its binding ability in a functional ELISA. When Recombinant Cynomolgus Monkey B7-H2 is coated at 0.5 μg/mL (100 μL/well), the concentration of Recombinant Cynomolgus Monkey ICOS Fc Chimera that produces 50% optimal binding response is 0.75-3.75 ng/mL.
Endotoxin
<0.10 EU/μg of the protein by the LAL method.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.
Notes
Disulfide-linked homodimer
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 500 μg/mL in PBS.
Bioactivity-ELISA 1
Bioactivity

Measured by its binding ability in a functional ELISA. When Recombinant Cynomolgus Monkey B7-H2 is coated at 0.5 μg/mL (100 μL/well), the concentration of Recombinant Cynomolgus Monkey ICOS Fc Chimera that produces 50% optimal binding response is 0.75-3.75 ng/mL.

Data Sheet MSDS
For research or industrial raw materials, not for personal medical use!

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