Recombinant Monkey ICOSLG Protein (Asp19-Thr256), C-6×His-tagged
Product Description
Cat
IMP-10424
Official Symbol
ICOSLG
Product Overview
Recombinant Cynomolgus Monkey B7-H2 protein (Asp19-Thr256) with a C-terminal 6×His tag was expressed in Human embryonic kidney cell.
Description
Enables identical protein binding activity. Predicted to be involved in T cell receptor signaling pathway and positive regulation of interleukin-4 production. Located in cytoplasmic ribonucleoprotein granule and plasma membrane.
Expression System
HEK293
Species
Monkey
Tag
C-6×His
Predicted N Terminal
Asp19
Form
Lyophilized from a 0.2 μm filtered solution in PBS.
Molecular Mass
Predicted Molecular Mass: 27 kDa
SDS-PAGE: 54-70 kDa, reducing conditions
Protein length
Asp19-Thr256
Bio-activity
Measured by its ability to co-stimulate IL-4 secretion by D10.G4.1 mouse helper T cells in the presence of anti-CD3. The ED50 for this effect is 0.15-0.9 μg/mL
Endotoxin
<0.10 EU/μg of the protein by the LAL method.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 200 μg/mL in PBS.
Bioactivity-ELISA 1
Bioactivity

Measured by its ability to co-stimulate IL-4 secretion by D10.G4.1 mouse helper T cells in the presence of anti-CD3. The ED50 for this effect is 0.15-0.9 μg/mL

Data Sheet MSDS
Datasheet MSDS
For research or industrial raw materials, not for personal medical use!

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