Recombinant Monkey LDLR Protein (Ala22-Gly788), C-6×His-tagged
Product Description
Cat
IMP-9645
Official Symbol
LDLR
Product Overview
Recombinant Cynomolgus Monkey LDLR protein (Ala22-Gly788) with a C-terminal 6×His tag was expressed in Human embryonic kidney cell.
Description
The low density lipoprotein receptor (LDLR) gene family consists of cell surface proteins involved in receptor-mediated endocytosis of specific ligands. The encoded protein is normally bound at the cell membrane, where it binds low density lipoprotein/cholesterol and is taken into the cell. Lysosomes release the cholesterol, which is made available for repression of microsomal enzyme 3-hydroxy-3-methylglutaryl coenzyme A (HMG CoA) reductase, the rate-limiting step in cholesterol synthesis. At the same time, a reciprocal stimulation of cholesterol ester synthesis takes place. Mutations in this gene cause the autosomal dominant disorder, familial hypercholesterolemia. Alternate splicing results in multiple transcript variants.
Expression System
HEK293
Species
Monkey
Tag
C-6×His
Predicted N Terminal
Ala22
Form
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Molecular Mass
Predicted Molecular Mass: 86 kDa
SDS-PAGE: 125-140 kDa, under reducing conditions.
Protein length
Ala22-Gly788
Bio-activity
Measured by its binding ability in a functional ELISA. When Human LDL is immobilized at 4.00 μg/mL (100 μL/well), Recombinant Cynomolgus Monkey LDLR His-tag binds with an ED50 of 30.0-300 ng/mL.
Endotoxin
<0.10 EU/μg of the protein by the LAL method.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 200 μg/mL in PBS.
SDS-PAGE
SDS-PAGE

Bioactivity-ELISA 1
Binding Activity

Measured by its binding ability in a functional ELISA. When Human LDL is immobilized at 4.00 μg/mL (100 μL/well), Recombinant Cynomolgus Monkey LDLR His-tag binds with an ED50 of 30.0-300 ng/mL.

Data Sheet MSDS
For research or industrial raw materials, not for personal medical use!

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