Recombinant Monkey LTBR Protein (Ser28-Met227), C-6×His-tagged
Product Description
Cat
IMP-9646
Official Symbol
LTBR
Product Overview
Recombinant Cyno Lymphotoxin beta R/TNFRSF3 protein (Ser28-Met227) with a C-terminal 6×His tag was expressed in Chinese Hamster Ovary cell line.
Description
This gene encodes a member of the tumor necrosis factor receptor superfamily. The major ligands of this receptor include lymphotoxin alpha/beta and tumor necrosis factor ligand superfamily member 14. The encoded protein plays a role in signalling during the development of lymphoid and other organs, lipid metabolism, immune response, and programmed cell death. Activity of this receptor has also been linked to carcinogenesis. Alternatively spliced transcript variants encoding multiple isoforms have been observed.
Expression System
CHO
Species
Monkey
Tag
C-6×His
Predicted N Terminal
Ser28 & Gln29 & Gln 31
Form
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Molecular Mass
Predicted Molecular Mass: 26 kDa
SDS-PAGE: 29-41 kDa, under reducing conditions.
Protein length
Ser28-Met227
Bio-activity
Measured by its ability to inhibit Recombinant Human Lymphotoxin alpha 1/ beta 2 induced IL-8 secretion in A375 human melanoma cells. The ED50 for this effect is 0.125-1.50 μg/mL.
Endotoxin
<0.10 EU/μg of the protein by the LAL method.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 500 μg/mL in PBS.
SDS-PAGE
SDS-PAGE

Bioactivity-ELISA 1
Bioactivity

Measured by its ability to inhibit Recombinant Human Lymphotoxin alpha 1/ beta 2 induced IL-8 secretion in A375 human melanoma cells. The ED50 for this effect is 0.125-1.50 μg/mL.

Data Sheet MSDS
For research or industrial raw materials, not for personal medical use!

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