Recombinant Monkey LTBR Protein (Ser28-Met227), C-hFc-tagged
Product Description
Cat
IMP-9625
Official Symbol
LTBR
Product Overview
Recombinant Cynomolgus Lymphotoxin beta R/TNFRSF3 protein (Ser28-Met227) with a Human IgG1 (Pro100-Lys330) Fc tag at C-terminus was expressed in Chinese Hamster Ovary cell line.
Description
This gene encodes a member of the tumor necrosis factor receptor superfamily. The major ligands of this receptor include lymphotoxin alpha/beta and tumor necrosis factor ligand superfamily member 14. The encoded protein plays a role in signalling during the development of lymphoid and other organs, lipid metabolism, immune response, and programmed cell death. Activity of this receptor has also been linked to carcinogenesis. Alternatively spliced transcript variants encoding multiple isoforms have been observed.
Expression System
CHO
Species
Monkey
Tag
C-hFc
Predicted N Terminal
Ser28
Form
Lyophilized from a 0.2 μm filtered solution in PBS.
Molecular Mass
Predicted Molecular Mass: 48 kDa
SDS-PAGE: 57-68 kDa, under reducing conditions
Protein length
Ser28-Met227
Bio-activity
Measured by its ability to inhibit Lymphotoxin alpha 1/beta 2-induced IL-8 secretion in A375 human melanoma cells. Degli-Esposti, M. et al. (1997) J. Immunol. 158:1756. The ED50 for this effect is 30.0-180 ng/mL in the presence of 100 ng/mL Recombinant Human Lymphotoxin alpha 1/beta 2.
Endotoxin
<0.10 EU/μg of the protein by the LAL method.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.
Notes
Disulfide-linked homodimer
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 500 μg/mL in PBS.
SDS-PAGE
SDS-PAGE

Bioactivity-ELISA 1
Bioactivity

Measured by its ability to inhibit Lymphotoxin alpha 1/beta 2-induced IL-8 secretion in A375 human melanoma cells. Degli-Esposti, M. et al. (1997) J. Immunol. 158:1756. The ED50 for this effect is 30.0-180 ng/mL in the presence of 100 ng/mL Recombinant Human Lymphotoxin alpha 1/beta 2.

Data Sheet MSDS
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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