Description
HGF R, also known as Met (from N-methyl-N’-nitro-N-nitrosoguanidine induced), is a glycosylated receptor tyrosine kinase that plays a central role in epithelial morphogenesis and cancer development. Based on the high homology (98%) between cynomolgus and human HGF R, cynomolgus HGF R is predicted to be synthesized as a single chain precursor which undergoes cotranslational proteolytic cleavage. This generates a mature HGF R that is a disulfide-linked dimer composed of a 50 kDa extracellular alpha chain and a 145 kDa transmembrane beta chain.
Molecular Mass
Predicted Molecular Mass: 128 kDa, 33 kDa ( alpha chain), and 96 kDa ( beta chain)
SDS-PAGE: 135-150 kDa, 40-45 kDa, and 103-115 kDa, under reducing conditions.
Bio-activity
Measured by its binding ability in a functional ELISA. When Recombinant Human HGF (NS0-expressed) Protein is immobilized at 0.250 μg/mL (100 μL/well), Recombinant Cynomolgus Monkey HGFR/c-MET Fc Chimera binds with an ED50 of 2.50-25.0 ng/mL.
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
