Recombinant Monkey NECTIN1 Protein (Gln31-Gly349), C-Fc-tagged
Product Description
Cat
IMP-9576
Official Symbol
NECTIN1
Product Overview
Recombinant Cynomolgus/Rhesus Macaque Nectin-1 protein (Gln31-Gly349) with a human IgG1 (Pro100-Lys330) Fc tag at C-terminus was expressed in Chinese Hamster Ovary cell line.
Description
This gene encodes an adhesion protein that plays a role in the organization of adherens junctions and tight junctions in epithelial and endothelial cells. The protein is a calcium(2+)-independent cell-cell adhesion molecule that belongs to the immunoglobulin superfamily and has 3 extracellular immunoglobulin-like loops, a single transmembrane domain (in some isoforms), and a cytoplasmic region. This protein acts as a receptor for glycoprotein D (gD) of herpes simplex viruses 1 and 2 (HSV-1, HSV-2), and pseudorabies virus (PRV) and mediates viral entry into epithelial and neuronal cells. Mutations in this gene cause cleft lip and palate/ectodermal dysplasia 1 syndrome (CLPED1) as well as non-syndromic cleft lip with or without cleft palate (CL/P). Alternative splicing results in multiple transcript variants encoding proteins with distinct C-termini.
Expression System
CHO
Species
Monkey
Tag
C-Fc
Predicted N Terminal
No results obtained: Gln31 predicted
Form
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Molecular Mass
Predicted Molecular Mass: 62 kDa
SDS-PAGE: 87-101 kDa, reducing conditions
Protein length
Gln31-Gly349
Bio-activity
Measured by its binding ability in a functional ELISA. When Recombinant Human Nectin-3 His-tag is immobilized at 1 μg/mL (100 μL/well), Recombinant Cynomolgus Monkey/Rhesus Macaque Nectin-1 Fc Chimera binds with an ED50 of 2.5-20 ng/mL.
Endotoxin
<0.10 EU/μg of the protein by the LAL method.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.
Notes
Disulfide-linked homodimer
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 1 mg/mL in PBS.
SDS-PAGE
SDS-PAGE

Bioactivity-ELISA 1
Binding Activity

Measured by its binding ability in a functional ELISA. When Recombinant Human Nectin-3 His-tag is immobilized at 1 μg/mL (100 μL/well), Recombinant Cynomolgus Monkey/Rhesus Macaque Nectin-1 Fc Chimera binds with an ED50 of 2.5-20 ng/mL.

Data Sheet MSDS
Datasheet MSDS
For research or industrial raw materials, not for personal medical use!

Online Inquiry
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  • 1-631-559-9269
    1-516-512-3133
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