Product Overview
Recombinant Cynomolgus Monkey PCSK9 protein, Gln31-Gln152 (pro domain) & Ser153-Gln692 (mature form), with a C-terminal 6×His tag was expressed in Chinese Hamster Ovary cell line.
Description
This gene encodes a member of the subtilisin-like proprotein convertase family, which includes proteases that process protein and peptide precursors trafficking through regulated or constitutive branches of the secretory pathway. The encoded protein undergoes an autocatalytic processing event with its prosegment in the ER and is constitutively secreted as an inactive protease into the extracellular matrix and trans-Golgi network. It is expressed in liver, intestine and kidney tissues and escorts specific receptors for lysosomal degradation. It plays a role in cholesterol and fatty acid metabolism. Mutations in this gene have been associated with autosomal dominant familial hypercholesterolemia. Alternative splicing results in multiple transcript variants.
Bio-activity
Measured by its binding ability in a functional ELISA. When Recombinant Human LDLR His-tag is immobilized at 1 μg/mL (100 μL/well), Recombinant Cynomolgus Monkey Proprotein Convertase 9/PCSK9 His-tag binds with an ED50 of 35-280 ng/mL.
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
6 months from date of receipt, -20 to -70 centigrade as supplied.
3 months, -20 to -70 centigrade under sterile conditions after opening.
