Recombinant Monkey PVRIG Protein (Gly20, Ser21 or Glu23-Leu172), C-hFc-tagged

Product Description

Cat

IMP-9622

Official Symbol

PVRIG

Product Overview

Recombinant Cynomolgus Monkey PVRIG protein (Gly20, Ser21 or Glu23-Leu172) with a Human IgG1 (Pro100-Lys330) Fc tag at C-terminus was expressed in Human embryonic kidney cell.

Description

Enables phosphatase binding activity and signaling receptor activity. Involved in negative regulation of T cell receptor signaling pathway. Located in plasma membrane.

Expression System

HEK293

Species

Monkey

Tag

C-hFc

Predicted N Terminal

Gly20, Ser21, Glu23

Form

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Molecular Mass

Predicted Molecular Mass: 45 kDa
SDS-PAGE: 52-63 kDa, under reducing conditions

Protein length

Gly20, Ser21 or Glu23-Leu172

Bio-activity

Measured by its binding ability in a functional ELISA. When Recombinant Cynomolgus Monkey PVRIG Fc Chimera is immobilized at 1.00 μg/mL (100 μL/well), Recombinant Human Nectin-2/CD112 binds with an ED50 of 0.150-0.750 μg/mL.

Endotoxin

<0.10 EU/μg of the protein by the LAL method.

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.

Notes

Disulfide-linked homodimer

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.

Reconstitution

Reconstitute at 500 μg/mL in PBS.

SDS-PAGE

Bioactivity-ELISA 1

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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