Recombinant Monkey RSPO3 Protein (Met33-His273)
Product Description
Cat
IMP-9629
Official Symbol
RSPO3
Product Overview
Recombinant Cynomolgus Monkey R-Spondin 3 protein (Met33-His273) without tag was expressed in Human embryonic kidney cell.
Description
This gene belongs to the R-spondin family. The encoded protein plays a role in the regulation of Wnt (wingless-type MMTV integration site family)/beta-catenin and Wnt/planar cell polarity (PCP) signaling pathways, which are involved in development, cell growth and disease pathogenesis. Genome-wide association studies suggest a correlation of this gene with bone mineral density and risk of fracture. This gene may be involved in tumor development.
Expression System
HEK293
Species
Monkey
Tag
Tag Free
Predicted N Terminal
Met33
Form
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Molecular Mass
Predicted Molecular Mass: 27 kDa
SDS-PAGE: 34-44 kDa, under reducing conditions.
Protein length
Met33-His273
Bio-activity
Measured by its ability to activate TCF reporter activity in HEK293 human embryonic kidney cells. The ED50 for this effect is 0.4-10 ng/mL.
Endotoxin
<0.10 EU/μg of the protein by the LAL method.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 250 μg/mL in PBS.
SDS-PAGE
SDS-PAGE

Bioactivity-ELISA 1
Binding Activity

Measured by its ability to activate TCF reporter activity in HEK293 human embryonic kidney cells. The ED50 for this effect is 0.4-10 ng/mL.

Data Sheet MSDS
For research or industrial raw materials, not for personal medical use!

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