Recombinant Monkey RSPO3 Protein (Met33-His273)
Product Description
Cat
IMP-9629
Official Symbol
RSPO3
Product Overview
Recombinant Cynomolgus Monkey R-Spondin 3 protein (Met33-His273) without tag was expressed in Human embryonic kidney cell.
Description
This gene belongs to the R-spondin family. The encoded protein plays a role in the regulation of Wnt (wingless-type MMTV integration site family)/beta-catenin and Wnt/planar cell polarity (PCP) signaling pathways, which are involved in development, cell growth and disease pathogenesis. Genome-wide association studies suggest a correlation of this gene with bone mineral density and risk of fracture. This gene may be involved in tumor development.
Expression System
HEK293
Species
Monkey
Tag
Tag Free
Predicted N Terminal
Met33
Form
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Molecular Mass
Predicted Molecular Mass: 27 kDa
SDS-PAGE: 34-44 kDa, under reducing conditions.
Protein length
Met33-His273
Bio-activity
Measured by its ability to activate TCF reporter activity in HEK293 human embryonic kidney cells. The ED50 for this effect is 0.4-10 ng/mL.
Endotoxin
<0.10 EU/μg of the protein by the LAL method.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 250 μg/mL in PBS.
SDS-PAGE
SDS-PAGE

Bioactivity-ELISA 1
Binding Activity

Measured by its ability to activate TCF reporter activity in HEK293 human embryonic kidney cells. The ED50 for this effect is 0.4-10 ng/mL.

Data Sheet MSDS
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

Online Inquiry
Contact Info