Recombinant Monkey TNFRSF4 Protein (Leu29-Ala216), C-6×His-tagged
Product Description
Cat
IMP-9566
Official Symbol
TNFRSF4
Product Overview
Recombinant Cynomolgus OX40/TNFRSF4 protein (Leu29-Ala216) with an C-terminal 6×His tag was expressed in Mouse myeloma cell line.
Description
The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor has been shown to activate NF-kappaB through its interaction with adaptor proteins TRAF2 and TRAF5. Knockout studies in mice suggested that this receptor promotes the expression of apoptosis inhibitors BCL2 and BCL2lL1/BCL2-XL, and thus suppresses apoptosis. The knockout studies also suggested the roles of this receptor in CD4+ T cell response, as well as in T cell-dependent B cell proliferation and differentiation.
Expression System
Mouse myeloma cell line
Species
Monkey
Tag
C-6×His
Predicted N Terminal
Leu29
Form
Lyophilized from a 0.2 μm filtered solution in PBS.
Molecular Mass
Predicted Molecular Mass: 21 kDa
SDS-PAGE: 40-55 kDa, under reducing conditions
Protein length
Leu29-Ala216
Bio-activity
Measured by its binding ability in a functional ELISA. When Recombinant Cynomolgus Monkey OX40/TNFRSF4 His tag is immobilized at 0.25 μg/mL (100 μL/well), Recombinant Human OX40 Ligand/TNFSF4 binds with an ED50 of 0.25-1.5 ng/mL.
Endotoxin
<0.10 EU/μg of the protein by the LAL method.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.
Reconstitution
Reconstitute at 500 μg/mL in PBS.
SDS-PAGE
SDS-PAGE

Bioactivity-ELISA 1
Binding Activity

Measured by its binding ability in a functional ELISA. When Recombinant Cynomolgus Monkey OX40/TNFRSF4 His tag is immobilized at 0.25 μg/mL (100 μL/well), Recombinant Human OX40 Ligand/TNFSF4 binds with an ED50 of 0.25-1.5 ng/mL.

Data Sheet MSDS
Datasheet MSDS
For research or industrial raw materials, not for personal medical use!

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