Recombinant Mouse Ace2 Protein (Gln18-Ser740), C-10×His-tagged
Product Description
Cat
IMP-10639
Official Symbol
Ace2
Product Overview
Recombinant Mouse ACE-2 protein (Gln18-Thr740) with a C-terminal 10-His tag was expressed in Chinese Hamster Ovary cell line.
Description
Predicted to enable identical protein binding activity; peptidase activity; and virus receptor activity. Involved in positive regulation of cardiac muscle contraction and tryptophan transport. Located in brush border membrane and cytoplasm. Is expressed in several structures, including brain; cardiovascular system; extraembryonic component; genitourinary system; and respiratory system. Used to study COVID-19 and congestive heart failure. Human ortholog(s) of this gene implicated in several diseases, including avian influenza; cerebral malaria; hypertension (multiple); respiratory syncytial virus infectious disease; and severe acute respiratory syndrome. Orthologous to human ACE2 (angiotensin converting enzyme 2).
Expression System
CHO
Species
Mouse
Tag
C-10×His
Predicted N Terminal
No results obtained: Gln18 predicted
Form
Supplied as a 0.2 μm filtered solution in Tris, NaCl and ZnCl2.
Molecular Mass
Predicted Molecular Mass: 85 kDa
SDS-PAGE: 110 kDa, reducing conditions
Protein length
Gln18-Ser740
Bio-activity
Measured by its ability to cleave a fluorogenic peptide substrate, Mca-YVADAPK(Dnp)-OH. The specific activity is >400 pmol/min/μg, as measured under the described conditions.
Endotoxin
<1.0 EU/μg of the protein by the LAL method.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.
Notes
Recombinant Mouse ACE-2 is prone to proteolytic cleavage at C-terminus. The predominant form of the purified protein lacks the His tag.
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
6 months from date of receipt, -20 to -70 centigrade as supplied.
3 months, -20 to -70 centigrade under sterile conditions after opening.
Bioactivity-ELISA 1
Enzyme Activity

Measured by its ability to cleave a fluorogenic peptide substrate, Mca-YVADAPK(Dnp)-OH. The specific activity is >400 pmol/min/μg, as measured under the described conditions.

Data Sheet MSDS
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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