Recombinant Mouse Adk Protein (1-361aa), N-His-Tagged
Product Description
Cat
IMP-9467
Official Symbol
Adk
Product Overview
Recombinant mouse Adk, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Description
Adk, also known as Adenosine kinase, is an abundant enzyme in mammalian tissues that catalyzes the transfer of the gamma-phosphate from ATP to adenosine, thereby serving as a regulator of concentrations of both extracellular adenosine and intracellular adenine nucleotides. Adenosine kinase has widespread effects on the cardiovascular, nervous, respiratory, and immune systems and inhibitors of the enzyme could play an important pharmacological role in increasing intravascular adenosine concentrations and acting as anti-inflammatory agents.
Expression System
E. coli
Species
Mouse
Tag
N-His
Form
Liquid in 20mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 50mM NaCl, 1mM EDTA
Molecular Mass
42.5 kDa (384 aa) confirmed by MALDI-TOF
Protein length
1-361
AA Sequence
MGSSHHHHHHSSGLVPRGSHMGSMAAADEPKPKKLKVEAPQALSENVLFGMGNPLLDISAVVDKDFLDKYSLKPNDQILAEDKHKELFDELVKKFKVEYHAGGSTQNSMKVAQWLIQEPHKAATFFGCIGIDKFGEILKRKAADAHVDAHYYEQNEQPTGTCAACITGGNRSLVANLAAANCYKKEKHLDLERNWVLVEKARVYYIAGFFLTVSPESVLKVARYAAENNRVFTLNLSAPFISQFFKEALMDVMPYVDILFGNETEAATFAREQGFETKDIKEIAKKAQALPKVNSKRQRTVIFTQGRDDTIVAAENDVTAFPVLDQNQEEIIDTNGAGDAFVGGFLSQLVSDKPLTECIRAGHYAASVIIRRTGCTFPEKPDFH
Endotoxin
< 1 EU/μg of protein (determined by LAL method)
Purity
> 95% by SDS-PAGE
Applications
Enzyme Activity,SDS-PAGE
Notes
Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.
Storage
Can be stored at +2 to +8 centigrade for 1 week. For long term storage, aliquot and store at -20 to -80 centigrade. Avoid repeated freezing and thawing cycles.
Concentration
1 mg/mL (determined by absorbance at 280nm)
SDS-PAGE
SDS-PAGE

3 μg by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

Data Sheet MSDS
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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